Salmonella mediated the hemagglutinating virus of Japan-envelope transfer suppresses tumor growth.
Salmonella mediated the hemagglutinating virus of Japan-envelope transfer suppresses tumor growth.
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DOI：
10.18632/oncotarget.17037
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发表时间：
2017-05-23
期刊：
影响因子：
--
通讯作者：
Kaneda Y
中科院分区：
文献类型：
--
作者：
Lee CH;Nishikawa T;Kaneda Y
沙门氏菌经过系统治疗后，可针对肿瘤微环境进行治疗。日本包膜血凝病毒(HVJ-E)诱导肿瘤细胞凋亡，对正常细胞无毒性。目前的HVJ-E治疗策略旨在利用HVJ-E进行肿瘤内治疗，在动物模型中显示出巨大的前景，但仅实现了有限的系统治疗。本研究的目的是研究用聚烯丙基胺盐酸盐(PAH)包被被命名为P-HVJ-E的微粒对HVJ-E抗肿瘤效果的调节作用。经P-HVJ-E处理后，血凝活性降低，维持肿瘤细胞选择性凋亡和抗肿瘤免疫功能。用沙门氏菌包被P-HVJ-E(PHS)，增强了其抗肿瘤活性，并保持了肿瘤靶向性。PHS治疗导致荷瘤小鼠肿瘤生长延迟。此外，对肿瘤的Western印迹分析表明，在PHS全身治疗后，HVJ-E靶向肿瘤。这些结果表明，沙门氏菌包被病毒颗粒可能为肿瘤的治疗提供一种新的途径。
Salmonella can target to tumor microenvironments after systemic treatment. The hemagglutinating virus of Japan-envelope (HVJ-E) induced apoptosis in tumor cells without toxicity in normal cells. Current HVJ-E therapeutic strategies, aimed at using HVJ-E for intratumor treatment, have shown great promise in animal models but have achieved only limited systemic treatment. The purpose of this study was to investigate the modulation of the anti-tumor efficiency of HVJ-E by coating the particles with poly (allylamine hydrochloride) (PAH), designated as P-HVJ-E. Treatment with P-HVJ-E resulted in decreased hemagglutinating activity and maintained tumor cell-selective apoptosis and anti-tumor immunity. The use of Salmonella as a coating for P-HVJ-E (PHS) enhanced the antitumor activity and maintained the tumor-targeting activity. Treatment with PHS resulted in delayed tumor growth in tumor-bearing mice. Furthermore, a Western blot assay of the tumors revealed that HVJ-E targeted to the tumor after systemic treatment with PHS. These results indicate that Salmonella coating viral particles may provide a new approach for tumor therapy.