Spatial and temporal variations in extracellular matrix of periocular and corneal regions during corneal stromal development.
Spatial and temporal variations in extracellular matrix of periocular and corneal regions during corneal stromal development.
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DOI：
10.1006/exer.1996.0033
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发表时间：
1996-03
影响因子：
3.4
通讯作者：
K. Doane;W. Ting;J. Mclaughlin;D. Birk
中科院分区：
文献类型：
--
作者：
K. Doane;W. Ting;J. Mclaughlin;D. Birk
鸟类角膜基质的发育发生在离散的发育阶段。在这一系列事件中，神经嵴来源的角膜成纤维细胞前体细胞被不同的细胞外基质包围，这些细胞外基质在空间和时间上都发生变化。为了阐明这些基质的作用，在眼周间充质和角膜中的细胞外基质成分进行了分析之前和期间的迁移和分化的角膜成纤维细胞使用抗体对胶原蛋白，蛋白多糖和糖蛋白。以前的工作集中在角膜基质的基质，而不是眼周间充质的基质。由于角膜成纤维细胞的前体存在于进入角膜之前必须通过眼周间充质迁移，因此对该环境进行了充分评价。本研究记录了角膜成纤维细胞前体开始角膜侵入之前和之后的发育阶段的角膜和眼周间充质的基质组成。包括眼周间充质和角膜的基质分子的变化被证明。这些变化包括I、II、III、IV和VI型胶原蛋白分布的变化;蛋白聚糖核心蛋白聚糖和光蛋白聚糖;以及粘附糖蛋白腱生蛋白、纤连蛋白和层粘连蛋白。据推测，基质定位的变化是重要的，在正常角膜发育过程中的细胞迁移和分化的调节。任何调节都可能涉及细胞外基质中发现的组分的组合，因此，需要考虑基质而不是孤立的组分。
The development of the avian corneal stroma occurs in discrete developmental stages. During this sequence of events, the neural crest-derived corneal fibroblast precursor cells are surrounded by distinct extracellular matrices which change both spatially and temporally. To elucidate the role of these matrices, extracellular matrix components in the periocular mesenchyme and cornea were analysed prior to and during migration and differentiation of corneal fibroblasts using antibodies against collagens, proteoglycans and glycoproteins. Previous work has concentrated on the matrix of the corneal stroma rather than the matrix of the periocular mesenchyme. Since the precursors of the corneal fibroblasts are present within the must migrate through the periocular mesenchyme prior to entry into the cornea proper, this environment was fully evaluated. The present study documents the matrix composition of both the cornea and periocular mesenchyme at developmental stages that are prior to and after initiation of corneal invasion by the corneal fibroblast precursors. Variations in matrix molecules comprising both the periocular mesenchyme and cornea were demonstrated. These include changes in the distribution of collagen types I, II, III, IV and VI; the proteoglycans decorin and lumican; as well as the adhesive glycoproteins tenascin, fibronectin and laminin. It is hypothesized that the variations in matrix localization are important in the regulation of cell migration and differentiation during normal corneal development. Any regulation is likely to involve a combination of components found in the extracellular matrices and therefore, a consideration of the matrix rather than isolated components is required.