Alveolar bone marrow as a cell source for regenerative medicine: Differences between alveolar and iliac bone marrow stromal cells
Alveolar bone marrow as a cell source for regenerative medicine: Differences between alveolar and iliac bone marrow stromal cells
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DOI：
10.1359/jbmr.041117
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发表时间：
2005-03-01
影响因子：
6.2
通讯作者：
Kato, Y
中科院分区：
文献类型：
--
作者：
Matsubara, T;Suardita, K;Kato, Y
我们从人牙槽骨/颌骨中分离并扩增BMSCs，成功率很高（70%）。这些细胞在体外和体内有强大的成骨潜力，虽然他们的软骨和脂肪形成的潜力是小于髂cells.Introduction：人骨髓基质细胞（BMSCs）具有成骨，软骨和脂肪形成的潜力，但骨髓抽吸髂嵴是一个侵入性的程序。牙槽骨骨髓间充质干细胞可能是更有用的再生medicine，因为骨髓可以从牙槽骨吸出最小的pain.Materials和方法：在这项研究中，牙槽骨骨髓样本从41例患者，6-66岁，在口腔外科手术过程中。将BMSC接种并维持在具有10%FBS和碱性成纤维细胞生长因子的培养物中。此外，骨髓基质细胞诱导分化成骨细胞，软骨细胞，或脂肪细胞在适当的medium.Results和结论：从一个小体积（0.1-3毫升）的吸出物，肺泡骨髓基质细胞扩增成功率为29/41（70%）。随着供体年龄的增加，成功率下降，这可能是因为年龄依赖性的骨髓间充质干细胞的数量和增殖能力下降。在成骨条件下，扩增的BMSC在21-28天分化为成骨细胞：骨钙素、骨桥蛋白和骨唾液酸蛋白的mRNA水平，沿着钙水平，在肺泡BMSC培养物中与在髂骨培养物中的那些相似。然而，与髂骨BMSC不同，牙槽骨BMSC显示出较差的软骨或脂肪形成潜力，并且在犬牙槽骨和髂骨BMSC之间观察到类似的差异。随后，将附着于P-磷酸三钙的人肺泡BMSCs移植到免疫缺陷小鼠中。在移植中，新骨由表达人波形蛋白、人骨钙素和人GAPDH的成骨细胞和骨细胞形成。这些结果表明，骨髓间充质干细胞具有独特的功能，这取决于他们在体内的位置和肺泡骨髓间充质干细胞将是有用的细胞治疗骨疾病。
We isolated and expanded BMSCs from human alveolar/jaw bone at a high success rate (70%). These cells had potent osteogenic potential in vitro and in vivo, although their chondrogenic and adipogenic potential was less than that of iliac cells.Introduction: Human bone marrow stromal cells (BMSCs) have osteogenic, chondrogenic, and adipogenic potential, but marrow aspiration from iliac crest is an invasive procedure. Alveolar BMSCs may be more useful for regenerative medicine, because the marrow can be aspirated from alveolar bone with minimal pain.Materials and Methods: In this study, alveolar bone marrow samples were obtained from 41 patients, 6-66 years of age, during the course of oral surgery. BMSCs were seeded and maintained in culture with 10% FBS and basic fibroblast growth factor. In addition, BMSCs were induced to differentiate into osteoblasts, chondrocytes, or adipocytes in appropriate medium.Results and Conclusion: From a small volume (0.1-3 ml) of aspirates, alveolar BMSCs expanded at a success ratio of 29/41 (70%). The success rate decreased with increasing donor age, perhaps because of age-dependent decreases in the number and proliferative capacity of BMSCs. The expanded BMSCs differentiated into osteoblasts under osteogenic conditions in 21-28 days: the mRNA levels of osteocalcin, osteopontin, and bone sialoprotein, along with the calcium level, in alveolar BMSC cultures were similar to those in iliac cultures. However, unlike iliac BMSC, alveolar BMSC showed poor chondrogenic or adipogenic potential, and similar differences were observed between canine alveolar and iliac BMSCs. Subsequently, human alveolar BMSCs attached to P-tricalcium phosphate were transplanted into immunodeficient mice. In transplants, new bone formed with osteoblasts and osteocytes that expressed human vimentin, human osteocalcin, and human GAPDH. These findings suggest that BMSCs have distinctive features depending on their in vivo location and that alveolar BMSCs will be useful in cell therapy for bone diseases.