Differences in in vivo distribution and homing of T cell subsets to mucosal vs nonmucosal lymphoid organs.
Differences in in vivo distribution and homing of T cell subsets to mucosal vs nonmucosal lymphoid organs.
复制标题
T 细胞亚群体内分布和归巢至粘膜与非粘膜淋巴器官的差异。
作者：
G. Kraal;I. Weissman;E. Butcher
本文研究了Lyt-2-和Lyt-2+T细胞在小鼠体内的迁移特性。在短期的体内归巢研究中，Lyt-2-T细胞在Peyer‘s斑块中的定位效率一致高于Lyt-2+T细胞(约1.5倍)，而两个群体在周围淋巴结中的定位大致相同。Lyt-2-和Lyt-2+亚群的这些归巢特性在很大程度上与它们的器官来源无关。迁移的特异性似乎是通过选择性识别高内皮微静脉(HEV)内皮细胞上的器官特异性决定因素来确定的，HEV是一种介导迁移淋巴细胞从血液中退出的特殊小静脉：在淋巴器官冰冻切片中淋巴细胞与HEV结合的体外分析中，Lyt-2细胞在与Peyer‘s斑块HEV结合的T细胞中所占的比例显著且一致地高于那些与外周结节HEV结合的T细胞。Lyt亚群的归巢和HEV识别偏好反映在它们在粘膜和非粘膜淋巴器官中的原位表达的差异，这表明这些群体的选择性迁移可能是决定局部免疫反应特征的一个重要因素。
The migratory properties of Lyt-2- and Lyt-2+ T cells in the mouse have been investigated. In short-term in vivo homing studies, Lyt-2- T cells localized consistently more efficiently than Lyt-2+ T cells in Peyer's patches (about 1.5 times as well), whereas both populations localized roughly equivalently in peripheral lymph nodes. These homing characteristics of Lyt-2- and Lyt-2+ subsets are largely independent of their organ source. The specificity of migration appears to be determined by selective recognition of organ-specific determinants on the endothelial cells of high endothelial venules (HEV), specialized venules that mediate the exit of migrating lymphocytes from the blood: In an in vitro assay of lymphocyte binding to HEV in lymphoid organ frozen sections, Lyt-2- cells constituted a significantly and consistently greater proportion of T cells binding to Peyer's patch HEV than of those binding to peripheral node HEV. The homing and HEV recognition preferences of the Lyt subsets are reflected in differences in their in situ representation in mucosal vs nonmucosal lymphoid organs, which suggests that the selective migration of these populations may be an important factor in determining the character of local immune responses.