Simultaneous quantitation of carbohydrate antigen 125 and carcinoembryonic antigen in human serum via time-resolved fluoroimmunoassay
Simultaneous quantitation of carbohydrate antigen 125 and carcinoembryonic antigen in human serum via time-resolved fluoroimmunoassay
复制标题
通过时间分辨荧光免疫分析同时定量人血清中的碳水化合物抗原 125 和癌胚抗原
DOI：
10.1016/j.cca.2018.05.003
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发表时间：
2018-08-01
影响因子：
5
通讯作者：
Liu, Tian-Cai
中科院分区：
文献类型：
--
作者：
Huang, Zhen;Zhai, Xiang-Ming;Liu, Tian-Cai
在肿瘤的临床诊断中，单一肿瘤标志物的免疫检测常常导致错误和漏检。诊断该病需要一种准确、灵敏、有效的定量方法。我们建立了一种双标记时间分辨荧光免疫分析法（TRFIA），同时检测人血清中碳水化合物抗原125 （CA125）和癌胚抗原（CEA），以帮助胃癌的诊断和预后。该方法基于微孔板夹心免疫分析法，以铕标记的抗ca125抗体和钐标记的抗cea抗体作为荧光报告。该方法检测范围广，检出限足以用于临床样品的检测。内、间变异系数均小于6%，加样回收率为90% ~ 110%。在血清样品中，双标记trfia和商业化学发光免疫测定在敏感性和特异性上没有显著的统计学差异。这些结果表明成功开发了一种有效、可靠、便捷的同时检测CA125和CEA的新型TRFIA方法，可用于临床血液筛查，监测肿瘤的发生发展，促进早期治疗。
In clinical diagnosis of cancer, immunology assay with single tumor marker often lead to a false and missed inspection. A quantitative method with a high degree of accuracy, sensitivity, and effectiveness is required for its diagnosis. We developed a dual-label time-resolved fluoroimmunoassay (TRFIA) to simultaneously detect carbohydrate antigen 125 (CA125) and carcinoembryonic antigen (CEA) in human serum to aid the diagnosis and prognosis of gastric cancer. The method was based on a microplate sandwich immunoassay using europium-labeled anti-CA125 antibodies and samarium-labeled anti-CEA antibodies as fluorescent reporters. The assay detection range was widely, and the limit of detection was sufficiently for detecting clinical sample. The intra- and inter-assay coefficients of variation were below 6%, and recoveries ranged from 90% to 110%. No significant statistical difference in sensitivity or specificity was observed between dual label-TRFIA and commercial chemiluminescent immunoassays in serum samples. These results demonstrate the successful development of an effective, reliable, and convenient novel TRFIA method for the simultaneous detection of CA125 and CEA, which can be used for clinical blood screening to monitor the occurrence and development of tumors to facilitate early treatment.