Isoproterenol-induced selective phosphorylation in vivo of the 214,000 dalton subunit of rat C6 glioma cell RNA polymerase II.
Isoproterenol-induced selective phosphorylation in vivo of the 214,000 dalton subunit of rat C6 glioma cell RNA polymerase II.
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异丙肾上腺素诱导大鼠 C6 神经胶质瘤细胞 RNA 聚合酶 II 214,000 道尔顿亚基体内选择性磷酸化。
DOI：
10.1016/0006-291x(81)91553-9
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发表时间：
1981
影响因子：
3.1
通讯作者：
R. Jungmann
中科院分区：
文献类型：
--
作者：
S. Lee;R. Jungmann
The phosphorylation in vivo of RNA polymerase II after isoproterenol stimulation of confluent rat C6 glioma cell cultures has been investigated. Glioma cells were incubated in the presence of Na 2 H 32 PO 4 and stimulated for 1 hour with the β-adrenergic agonist isoproterenol. The phosphorylation pattern was analyzed after purification of RNA polymerase II by immunoprecipitation, SDS-polyacrylamide gel electrophoresis and autoradiography. Isoproterenol markedly increased [32 P] phosphate incorporation into the 214,000 dalton RNA polymerase subunit. Analysis of the phosphate acceptor amino acid revealed the presence of only [32 P] phosphoserine. The data demonstrates an isoproterenol-induced structural modification of RNA polymerase II.
