Tutorial: measurement of fluorescence spectra and determination of relative fluorescence quantum yields of transparent samples
Tutorial: measurement of fluorescence spectra and determination of relative fluorescence quantum yields of transparent samples
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DOI：
10.1088/2050-6120/ab7e10
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发表时间：
2020-07-01
影响因子：
3.2
通讯作者：
Levitus, Marcia
中科院分区：
文献类型：
--
作者：
Levitus, Marcia
透明样品中荧光光谱的测量和荧光量子产率的测定在概念上是简单的任务，但这些程序受到几个陷阱的影响，这些陷阱可能会导致显着的错误。现有的技术报告和协议通常假设读者拥有坚实的光谱学理论背景，并有丰富的经验与荧光仪器，但这往往不是这种情况下，许多应用荧光在不同的科学领域。本教程的目标是提供一个教学治疗的主题，希望将访问读者没有广泛的专业知识，在荧光领域。这篇文章涵盖了理解研究人员所能期望的最常见的人工制品的起源所需的理论背景。可能的工件用例子来说明，以帮助读者避免它们或识别它们（如果存在）。一步一步的例子，在溶液中的荧光量子产率测定提供了详细的实验信息，以帮助读者了解如何设计和分析实验。
The measurement of fluorescence spectra and the determination of fluorescence quantum yields in transparent samples are conceptually simple tasks, but these procedures are subject to several pitfalls that can lead to significant errors. Available technical reports and protocols often assume that the reader possesses a solid theoretical background in spectroscopy and has ample experience with fluorescence instrumentation, but this is often not the case given the many applications of fluorescence in diverse fields of science. The goal of this tutorial is to provide a didactic treatment of the topic that will hopefully be accessible to readers without extensive expertise in the field of fluorescence. The article covers the theoretical background needed to understand the origins of the most common artifacts researchers can expect. Possible artifacts are illustrated with examples to help readers avoid them or identify them if present. A step-by-step example of a fluorescence quantum yield determination in solution is provided with detailed experimental information to help readers understand how to design and analyze experiments.