Evaluation of phenolic acids and phenylpropanoids in the crude drugs
Evaluation of phenolic acids and phenylpropanoids in the crude drugs
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DOI：
10.3390/medicina44010008
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发表时间：
2008-01-01
影响因子：
2.6
通讯作者：
Baranauskas, Algirdas
中科院分区：
文献类型：
--
作者：
Ivanauskas, Liudas;Jakstas, Valdas;Baranauskas, Algirdas
酚酸和苯丙素具有重要的生物活性，是天然药物的治疗成分。开发这些植物治疗成分的有效分析技术（指纹图谱和含量测定方法）对于草药制剂的功效、安全性和质量控制是一项重要的实践。本研究的目的是研究对选定的酚酸和苯丙素（咖啡酸、绿原酸、肉桂酸、香豆酸、阿魏酸、没食子酸、原儿茶酸（3,4 - 二羟基苯甲酸）、迷迭香酸、香草酸和香草醛）进行评估的分析能力。对反相高效液相色谱快速简便方法进行了优化和验证。优化后的色谱方法的流动相由甲醇和水中0.5%的乙酸溶液组成。为了应用该方法，选择了两种原料：蜂胶和牛至草。香豆酸是蜂胶中主要的酚酸（2785微克/克）。对牛至草的分析结果表明，样品中迷迭香酸和原儿茶酸含量较高，分别为（6376微克/克）和（1485微克/克）。
Phenolic acids and phenylpropanoids have an important biological activity and are therapeutic agents of crude drugs. Development of validated analysis techniques of these phytotherapeutic agents (fingerprinting and assay procedures) is an important practice for efficacy, safety, and quality control of herbal drug preparations. The aim of the present work was to study analytical capabilities of the evaluation of selected phenolic acids and phenylpropanoids: caffeic acid, chlorogenic acid, cinnamic acid, coumaric acid, ferulic acid, gallic acid, protocatechuic (3,4-dihydroxybenzoic) acid, rosmarinic acid, vanillic acid, and vanillin. Optimization and validation procedures of rapid and simple method of reversed-phase high-performance liquid chromatography were carried out. The mobile phase of the optimized chromatographic method consisted of methanol and 0.5% acetic acid solvent in water. For the application of method, two kinds of raw materials were chosen: propolis and the Herba Origani. Coumaric acid is the dominating phenolic acid of propolis (2785 mu g/g). Results of analysis of Herba Origani demonstrated high quantities (63 76 mu g/g) of rosmarinic and protocatechuic (1485 mu g/g) acids in the samples.