Development and optimization of an immunoassay for the detection of Hg(II) in lake water
Development and optimization of an immunoassay for the detection of Hg(II) in lake water
复制标题
湖水中 Hg(II) 检测免疫分析的开发和优化
DOI：
10.1002/fsn3.991
复制
发表时间：
2019-05-01
影响因子：
3.9
通讯作者：
Yuan, Jian
中科院分区：
文献类型：
--
作者：
Jin, Xuexue;He, Rong;Yuan, Jian
本文开发并优化了一种基于单克隆抗体 (mAb-A24) 的间接竞争酶联免疫吸附测定 (IC-ELISA)，用于检测自来水和湖水中的汞 (II)。在优化过程中对一些稳定添加剂（明胶、牛血清白蛋白[BSA]、聚乙烯醇[PVA]和聚乙烯吡咯烷酮[PVP]）和表面活性剂（Tween-20）进行了深入研究。在最佳条件下，50%半数抑制浓度（IC50）和检测限（LOD）分别为1.68和0.079 ng/ml。这些抗 Hg mAb 还与甲基汞 (CH3Hg) 有一定的亲和力，并且与其他十三种金属离子没有交叉反应性。所开发的方法对自来水和湖水中的汞 (II) 的回收率令人满意，范围在 91% 至 116% 之间。因此，该免疫分析可用于检测环境水中的痕量Hg(II)。
In this paper, an indirect competitive enzyme-linked immunosorbent assay (IC-ELISA) has been developed and optimized to detect Hg(II) in tap water and lake water based on a monoclonal antibody (mAb-A24). Some stabilizing additives (Gelatin, bovine serum albumin [BSA], polyvinyl alcohol [PVA], and polyvinyl pyrrolidone [PVP]) and surfactant (Tween-20) have been investigated thoroughly in the optimization process. Under the optimal condition, the 50% half maximal inhibitory concentration (IC50) and limit of detection (LOD) were 1.68 and 0.079 ng/ml, respectively. These anti-Hg mAbs also have some affinity with methyl mercury (CH3Hg) and with no cross-reactivity with other thirteen metal ions. The developed method has shown satisfactory recovery of Hg(II), ranged between 91% and 116%, from tap water and lake water. Therefore, this immunoassay can be used to detect trace Hg(II) in environment water.