Graphene Oxide-Silver Nanocomposite: Novel Agricultural Antifungal Agent against Fusarium graminearum for Crop Disease Prevention
Graphene Oxide-Silver Nanocomposite: Novel Agricultural Antifungal Agent against Fusarium graminearum for Crop Disease Prevention
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氧化石墨烯-银纳米复合材料：针对禾谷镰刀菌的新型农用抗真菌剂，用于预防作物病害
DOI：
10.1021/acsami.6b05730
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发表时间：
2016-09-14
影响因子：
9.5
通讯作者：
Han, Heyou
中科院分区：
文献类型：
--
作者：
Chen, Juanni;Sun, Long;Han, Heyou
基于纳米颗粒的抗菌剂已经成为一个涉及医学，材料科学，生物学和化学的跨学科领域，因为它们的尺寸依赖性质量，高表面积与体积比和独特的理化性质。其中一些化合物在植物保护和营养方面的应用前景广阔。本文首次采用界面静电自组装法制备了GO-AgNPs纳米复合材料，并研究了GO-AgNPs纳米复合材料对植物病原菌禾谷镰刀菌（Fusarium graminearum）的体内外抑菌活性。结果表明：GO-AgNPs纳米复合物显示出比纯AgNPs、添加GO悬浮液分别增加几乎3倍和7倍的抑制效率。4.68 μ g/mL（最低抑菌浓度（MIC））的相对低浓度对孢子萌发有抑制作用。孢子和菌丝被破坏，这可能是由于GO-AgNPs的显著协同作用引起的抗菌机制，诱导物理损伤和化学活性氧的产生。更重要的是，化学。真菌孢子介导的GO分泌可能是GO-AgNPs具有较高抗菌活性的原因。此外，GO-AgNPs纳米复合材料对番茄叶斑病的防治效果显著。在离体叶片实验中，本研究的所有结果表明，在这项工作中开发的GO-AgNPs纳米复合材料具有作为开发针对病原真菌或细菌的新型抗菌剂的有前途的材料的潜力。
Nanoparticle-based antibacterial agents have emerged as an interdisciplinary field involving medicine, material science, biology, and chemistry because of their size-dependent qualities, high surface-to-volume ratio, and unique physiochemical properties. Some of them have shown great promise for their application in plant protection and nutrition. Here, GO-AgNPs nanocomposite was fabricated through interfacial electrostatic self assembly and its antifungal activity against phytopathogen Fusarium graminearum was investigated in vitro and in vivo-foe the first time. The results demonstrated that the:GO-AgNPs nanocomposite showed almost a 3- and 7-fold increase of inhibition efficiency over pure AgNPs, add GO suspension, respectively. The spore germination inhibition was stimulated by relatively low concentration of 4.68 mu g/mL (minimum inhibition-concentration (MIC)). The spores and hyphae were damaged, which might be caused by an antibacterial: mechanism from the remarkable synergistic effect of GO-AgNPs, inducing physical injury and chemical reactive oxygen species generation. More importantly, the,chemical. eduction of GO mediated by fungal spores was possibly contributed to the high antimicrobial activity Of GO-AgNPs. Furthermore, the GO-AgNPs nanocomposite showed a significant effect in controlling the leaf spot disease infected by F. graminearum in the detached leaf experiment. All the results from this research suggest that the GO-AgNPs nanocomposite developed in this work has the potential as a promising material for the development of novel antimicrobial agents against pathogenic fungi or bacteria.