A Sulfhydryl Reagent Modulates Systemic Signaling for Wound-Induced and Systemin-Induced Proteinase Inhibitor Synthesis
A Sulfhydryl Reagent Modulates Systemic Signaling for Wound-Induced and Systemin-Induced Proteinase Inhibitor Synthesis
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DOI：
10.1104/pp.105.2.725
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发表时间：
1994-06
期刊：
影响因子：
4.3
通讯作者：
J. Narváez-Vásquez;M. Orozco-Cárdenas;C. Ryan
中科院分区：
文献类型：
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作者：
J. Narváez-Vásquez;M. Orozco-Cárdenas;C. Ryan
巯基试剂对氯癸基苯磺酸（PCMBS），在几种植物中的蔗糖的活性质外体韧皮部加载的一个既定的抑制剂，被证明是一个强大的抑制剂的伤口诱导和系统素诱导的激活蛋白酶抑制剂的合成和积累在番茄植物（番茄品种Castlemart）的叶子。PCMBS，提供给年轻的番茄植物通过其切割茎，块积累的蛋白酶抑制剂在叶中响应受伤。将系统素直接应用于新鲜伤口可增强蛋白酶抑制剂的全身积累，使其水平高于单独的创伤。将PCMBS放置在新鲜伤口上，在存在和不存在外源性系统素的情况下，都会严重抑制蛋白酶抑制剂的全身诱导。半胱氨酸、二硫苏糖醇和谷胱甘肽可逆转PCMBS抑制。放射性标记的系统素放在新鲜的伤口很容易从受伤的叶片运输到上部叶片。然而，在存在PCMBS的情况下，放射性标记的系统素不会从伤口部位转运走。诱导蛋白酶抑制剂I的合成oliguronides（聚合度[几乎等于] 20），亚麻酸，或茉莉酸甲酯没有抑制PCMBS。累积的数据支持巯基在介导系统素从伤口部位易位到番茄植物的远端受体位点中可能发挥的作用，并进一步支持系统素作为系统性伤口信号的作用。
The sulfhydryl group reagent p-chloromecuribenzene sulfonic acid (PCMBS), an established inhibitor of active apoplastic phloem loading of sucrose in several plant species, is shown to be a powerful inhibitor of wound-induced and systemin-induced activation of proteinase inhibitor synthesis and accumulation in leaves of tomato plants (Lycopersicon esculentum cv Castlemart). PCMBS, supplied to young tomato plants through their cut stems, blocks accumulation of proteinase inhibitors in leaves in response to wounding. The application of systemin directly to fresh wounds enhances systemic accumulation of proteinase inhibitors to levels higher than wounding alone. Placed on fresh wounds, PCMBS severely inhibits systemic induction of proteinase inhibitors, in both the presence and absence of exogenous systemin. PCMBS inhibition can be reversed by cysteine, dithiothreitol, and glutathione. Radiolabeled systemin placed on fresh wounds is readily transported from the wounded leaves to upper leaves. However, in the presence of PCMBS, radiolabeled systemin is not transported away from wound sites. Induction of proteinase inhibitor I synthesis by oligouronides (degree of polymerization [almost equal to] 20), linolenic acid, or methyl jasmonate was not inhibited by PCMBS. The cumulative data support a possible role for sulfhydryl groups in mediating the translocation of systemin from wound sites to distal receptor sites in tomato plants and further support a role for systemin as a systemic wound signal.