Programmable One-Pot Multistep Organic Synthesis Using DNA Junctions
Programmable One-Pot Multistep Organic Synthesis Using DNA Junctions
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DOI：
10.1021/ja2101196
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发表时间：
2012-01-25
影响因子：
15
通讯作者：
Turberfield, Andrew J.
中科院分区：
文献类型：
--
作者：
McKee, Mireya L.;Milnes, Phillip J.;Turberfield, Andrew J.
多步DNA模板合成系统由DNA连接的顺序形成控制。将反应物连接到DNA衔接子上，所述衔接子通过与DNA模板链杂交而聚集在一起。可以重复该过程以允许序列控制的低聚物合成，同时在每个反应步骤保持恒定的反应环境，而与低聚物长度无关。合成可以在含有所有所需反应性单体的单锅中进行。不同的低聚物可以在同一容器中平行合成，并且平行合成的产物可以连接，从而减少产生给定长度的低聚物所需的反应步骤的数量。
A system for multistep DNA-templated synthesis is controlled by the sequential formation of DNA junctions. Reactants are attached to DNA adapters which are brought together by hybridization to DNA template strands. This process can be repeated to allow sequence-controlled oligomer synthesis while maintaining a constant reaction environment, independent of oligomer length, at each reaction step. Synthesis can take place in a single pot containing all required reactive monomers. Different oligomers can be synthesized in parallel in the same vessel, and the products of parallel synthesis can be ligated, reducing the number of reaction steps required to produce an oligomer of a given length.