Multiple astrovirus MLB1, MLB2, VA2 clades, and classic human astrovirus in children with acute gastroenteritis in Japan
Multiple astrovirus MLB1, MLB2, VA2 clades, and classic human astrovirus in children with acute gastroenteritis in Japan
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DOI：
10.1002/jmv.24337
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发表时间：
2016-02-01
影响因子：
12.7
通讯作者：
Ushijima, Hiroshi
中科院分区：
文献类型：
--
作者：
Khamrin, Pattara;Thongprachum, Aksara;Ushijima, Hiroshi
这项研究调查了2012年至2013年日本急性胃肠炎儿童中星状病毒的遗传变异性。使用RT-PCR和核苷酸测序技术对330例腹泻儿童的粪便样本进行了星状病毒检测。在检测的标本中，有54例(16.4%)呈星状病毒阳性。检测到三个不同的系统发育分支。MLB是最主要的分支(35个样本)，其次是经典星状病毒(17个样本)和VA星状病毒(2个样本)。鉴定出MLB的两种类型(MLB1和MLB2)，而经典的星状病毒是HAstV1和HAstV4。经鉴定，两株VA星状病毒为VA2。(C)2015年威利期刊公司。
This study investigated the genetic variability of astroviruses in children with acute gastroenteritis in Japan in 2012 to 2013. A total of 330 fecal specimens collected from children with diarrhea were tested for astroviruses using RT-PCR and nucleotide sequencing techniques. Among the tested specimens, 54 (16.4%) were positive for astrovirus. Three different phylogenetic clades were detected. MLB was the most predominant clade (35 samples), followed by classic astrovirus (17 samples), and VA astrovirus (2 samples). Two types of MLB were identified (MLB1 and MLB2), while classic astroviruses were HAstV1 and HAstV4. Two VA astroviruses were identified as VA2. (c) 2015 Wiley Periodicals, Inc.