CHANGE OF BIOLOGICAL-ACTIVITIES OF (1-]3)-BETA-D-GLUCAN FROM GRIFOLA-FRONDOSA UPON MOLECULAR-WEIGHT REDUCTION BY HEAT-TREATMENT
CHANGE OF BIOLOGICAL-ACTIVITIES OF (1-]3)-BETA-D-GLUCAN FROM GRIFOLA-FRONDOSA UPON MOLECULAR-WEIGHT REDUCTION BY HEAT-TREATMENT
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DOI：
10.1248/cpb.38.477
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发表时间：
1990-02-01
影响因子：
1.7
通讯作者：
YADOMAE, T
中科院分区：
文献类型：
--
作者：
ADACHI, Y;OHNO, N;YADOMAE, T
生物活性的变化表现为（1 →）6)-分支的（1 →3）-β-通过热处理相应的完整β-葡聚糖而获得的各种分子量的D-葡聚糖，150 ℃下的葡聚糖C（HD-LE）。本研究中评估的活性如下：抗肿瘤活性、旁路补体途径激活、巨噬细胞的葡萄糖消耗、培养上清液和细胞裂解物中巨噬细胞介导的溶酶体酶活性、白细胞介素-1（IL-1）活性和佐剂活性。HD-LE可分为三组：1）HD-LE 0 h（MW 800000），其激活所有测试的生物活性，2）HD-LE 0.5和3 h（MW 250000和21000），其缺乏或表现出低水平的活性，例如替代补体途径和溶酶体酶分泌的活化，（3）HD-LE 6 h（MW 6400）仅激活葡萄糖消耗和溶酶体酶的合成。这些结果表明，抗肿瘤葡聚糖并不总是宿主防御机制的多重增强剂，并且需要大分子量来增强多种免疫活性。
Changes of biological activities manifested by (1 .fwdarw. 6)-branched (1 .fwdarw. 3)-.beta.-D-glucans of various molecular weights obtained by heat treatment of the corresponding intact .beta.-glucan at 150.degree. C (HD-LE) were examined. The activities assessed in this study were as follows: an antitumor activity, activation of alternative complement pathway, glucose consumption by macrophages, macrophage-mediated lysosomal enzyme activity in culture supernatant and cell lysate, interleukin-1 (IL-1) activity, and adjuvant activity. HD-LE could be classified into three groups: 1) HD-LE 0 h (MW 800000) which activated all of the biological activities tested, 2) HD-LE 0.5 and 3 h (MW 250000 and 21000) which lacked or exhibited low levels of activities such as activation of alternative complement pathway and lysosomal enzyme secretion, 3) HD-LE 6 h (MW 6400) which only activated glucose consumption and synthesis of lysosomal enzyme. These results suggest that an antitumor glucan is not always a multiple enhancer of host defense mechanisms and that a large molecular weight is required to augment multiple immunological activities.