Parasitization by Scleroderma guani influences expression of superoxide dismutase genes in Tenebrio molitor.
Parasitization by Scleroderma guani influences expression of superoxide dismutase genes in Tenebrio molitor.
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DOI：
10.1002/arch.21179
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发表时间：
2014-09
影响因子：
2.2
通讯作者：
Jiaying Zhu;Sangzi Ze;D. Stanley;Bin Yang
中科院分区：
文献类型：
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作者：
Jiaying Zhu;Sangzi Ze;D. Stanley;Bin Yang
超氧化物歧化酶（SOD）是一种参与活性氧解毒的抗氧化酶。在本研究中，我们鉴定了黄粉虫甲虫细胞外和细胞内铜锌SOD （ecCuZnSOD和icCuZnSOD）和锰SOD （MnSOD）的编码基因。ecCuZnSOD、icCuZnSOD和MnSOD分别编码24.55、15.81和23.14 kDa的多肽，具有其他昆虫sod的典型结构特征。与家蚕、家蝇、玻璃鼻虫、人弓根和木栗虫的SOD序列具有20 ~ 94%的同源性。分析了这些基因在特定组织和发育阶段的表达，以及暴露于大肠杆菌和被瓜硬皮病寄生后的表达。我们记录了这三种sod在角质层、脂肪体和血细胞以及主要发育阶段的表达。与其他发育阶段相比，在后期幼虫和蛹中检测到相对较高的表达。细菌感染后，转录水平上调。对寄生蛹的分析发现，寄生后显著诱导了T. molitor SOD基因的表达。我们推断这些基因在免疫反应和宿主-寄生虫相互作用中起作用。
Superoxide dismutase (SOD) is an antioxidant enzyme involved in detoxifying reactive oxygen species. In this study, we identified genes encoding the extracellular and intracellular copper-zinc SODs (ecCuZnSOD and icCuZnSOD) and a manganese SOD (MnSOD) in the yellow mealworm beetle, Tenebrio molitor. The cDNAs for ecCuZnSOD, icCuZnSOD, and MnSOD, respectively, encode 24.55, 15.81, and 23.14 kDa polypeptides, which possess structural features typical of other insect SODs. They showed 20-94% identity to other known SOD sequences from Bombyx mori, Musca domestica, Nasonia vitripennis, Pediculus humanus corporis, and Tribolium castaneum. Expression of these genes was analyzed in selected tissues and developmental stages, and following exposure to Escherichia coli and parasitization by Scleroderma guani. We recorded expression of all three SODs in cuticle, fat body, and hemocytes and in the major developmental stages. Relatively higher expressions were detected in late-instar larvae and pupae, compared to other developmental stages. Transcriptional levels were upregulated following bacterial infection. Analysis of pupae parasitized by S. guani revealed that expression of T. molitor SOD genes was significantly induced following parasitization. We infer that these genes act in immune response and in host-parasitoid interactions.