Steady-state and lifetime-resolved fluorescence-detected circular dichroism using a modified phase-modulation spectrofluorometer
Steady-state and lifetime-resolved fluorescence-detected circular dichroism using a modified phase-modulation spectrofluorometer
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使用改进的相位调制分光荧光计进行稳态和寿命分辨荧光检测圆二色性
DOI：
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发表时间：
1993
期刊：
影响因子：
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通讯作者：
L. McGown
中科院分区：
文献类型：
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作者：
Karen Wu;L. Geng;M. J. Joseph;L. McGown
本文介绍了一种新的仪器方法，荧光检测圆二色性（FD-CD），也允许前所未有的分辨率的圆二色性的混合物中的每一个组成部分，通过一种新的技术，寿命分辨荧光检测圆二色性（LRFDCD）。FDCD和LRFDCD都是在商业相位调制荧光分光光度计上进行的，该分光光度计被修改以产生右旋和左旋圆偏振激发光。这些仪器的修改进行了说明，包括讨论的标准，选择巴比内-索莱补偿器作为偏振器件
This paper describes a new instrumental approach to fluorescence-detected circular dichroism (FD-CD) that also allows unprecedented resolution of the circular dichroism of each component in mixtures through a new technique, lifetime-resolved fluorescence-detected circular dichroism (LRFDCD). Both FDCD and LRFDCD are performed on a commercial phase-modulation spectrofluorometer that is modified to produce right-handed and left-handed circularly polarized exciting light. These instrumental modifications are described, including a discussion ofthe criteria for selection of the Babinet-Soleil compensator as the polarization device