Nbseptin2 Expression Pattern and Its Interaction with NbPTP1 during Microsporidia Nosema bombycis Polar Tube Extrusion
Nbseptin2 Expression Pattern and Its Interaction with NbPTP1 during Microsporidia Nosema bombycis Polar Tube Extrusion
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家蚕微孢子虫极管挤出过程中 Nbseptin2 的表达模式及其与 NbPTP1 的相互作用
DOI：
10.1111/jeu.12752
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发表时间：
2019-09-12
影响因子：
2.2
通讯作者：
Zhou, Zeyang
中科院分区：
文献类型：
--
作者：
Liu, Fangyan;Chen, Jie;Zhou, Zeyang
家蚕微孢子虫（Nosemabombycis，Nb）是一种能引起家蚕微粒子病的微孢子虫，给蚕业生产造成了严重损失。萌发是微孢子虫入侵过程中的重要生物学事件。Septins是一个膜相关蛋白家族，在组织入侵中起着关键作用，并且已被认为是许多病原体中的毒力因子。本实验室的前期工作表明，家蚕微孢子虫septin2（Nbseptin2）与枯草杆菌蛋白酶样蛋白酶2（NbSLP2）相互作用。在此，我们发现Nbseptin2在孢子中主要与质膜结合。孢子萌发后，发现Nbseptin2与极管蛋白1（NbPTP 1）共定位于极帽和极管近端区。免疫共沉淀和酵母双杂交分析进一步证实了Nbseptin2与NbPTP 1相互作用。Nbseptin2在孢子中的移位和相互作用表明Nbseptin2可能在微孢子虫极管挤出过程中起重要作用。我们的发现提高了对微孢子虫萌发机制的理解。
Nosema bombycis (Nb) is a deadly species of microsporidia capable of causing pebrine, leading to heavy losses in sericulture. Germination is an important biological event in the invasion process of microsporidia. Septins, a family of membrane-associated proteins, play a critical role in tissue invasion and have been recognized as a virulence factor in numerous pathogens. Previous work in our laboratory has shown that Nosema bombycis septin2 (Nbseptin2) interacts with subtilisin-like protease 2 (NbSLP2). Herein, we found that Nbseptin2 was mainly associated with the plasma membrane in spores. Following spore germination, Nbseptin2 was found to co-localize with polar tube protein 1 (NbPTP1) at the polar cap and proximal zone of the polar tube. Co-immunoprecipitation and yeast two-hybrid analysis further confirmed that Nbseptin2 interacted with NbPTP1. The translocation and interaction of Nbseptin2 in the spores suggest that Nbseptin2 may play a significant role in microsporidia polar tube extrusion process. Our findings improve understanding of the mechanisms underlying microsporidia germination.