Effect of the antibiotic azithromycin on thermotropic behavior of DOPC or DPPC bilayers
Effect of the antibiotic azithromycin on thermotropic behavior of DOPC or DPPC bilayers
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DOI：
10.1016/j.chemphyslip.2006.08.002
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发表时间：
2006-10-01
影响因子：
3.4
通讯作者：
Mingeot-Leclercq, M. -P.
中科院分区：
文献类型：
--
作者：
Fa, N.;Ronkart, S.;Mingeot-Leclercq, M. -P.
阿奇霉素是一种大环内酯类抗生素，已知与脂质结合并可能通过与脂质膜相互作用影响内吞作用[Tyteca，D.，Schanck，A.，Dufrene，Y.F.，Deleu，M.，Courtoy，P.J.，Tulkens，P.M.，Mingeot-Leclercq，MP，2003.大环内酯类抗生素阿奇霉素与脂质相互作用并影响膜组织和流动性：对Langmuir-Blodgett单层、脂质体和J774巨噬细胞的研究。J. 192，203-215]：在这项工作中，我们研究了阿奇霉素对由1，2-二油酰-sn-甘油-3-磷酸胆碱（DOPC）或1，2-二棕榈酰-sn-甘油-3-磷酸胆碱（DPPC）制成的脂质模型膜的影响。用P-31 NMR和DSC研究了两种脂质在与阿奇霉素接触时的热转变。关于DPPC，阿奇霉素诱导抑制的pretransition，而观察到的DOPC和抗生素之间的相分离。对于这两种脂质，与相变相关的焓强烈降低与阿奇霉素。这种效应可能是由于阿奇霉素插入脂质后，疏水链之间的可用空间增加。该发现提供了由阿奇霉素诱导的DOPC流体基质中DPPC凝胶相合并的分子见解[Berquand，A.，Mingeot-Leclercq，MP，Dufrene，YE，2004年。原子力显微镜对药物-膜相互作用的实时成像。生物化学生物Biophys. Acta 1664，198-205]，并且可以帮助更好地理解阿奇霉素-细胞相互作用。(c)2006爱思唯尔爱尔兰有限公司保留所有权利。
Azithromycin is a macrolide antibiotic known to bind to lipids and to affect endocytosis probably by interacting with lipid membranes [Tyteca, D., Schanck, A., Dufrene, Y.F., Deleu, M., Courtoy, P.J., Tulkens, P.M., Mingeot-Leclercq, M.P., 2003. The macrolide antibiotic azithromycin interacts with lipids and affects membrane organization and fluidity: studies on Langmuir-Blodgett monolayers, liposomes and J774 macrophages. J. Membr. Biol. 192, 203-215]: In this work, we investigate the effect of azithromycin on lipid model membranes made of 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) or 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC). Thermal transitions of both lipids in contact with azithromycin are studied by P-31 NMR and DSC on multilamellar vesicles. Concerning the DPPC, azithromycin induces a suppression of the pretransition whereas a phase separation between the DOPC and the antibiotic is observed. For both lipids, the enthalpy associated with the phase transition is strongly decreased with azithromycin. Such effects may be due to an increase of the available space between hydrophobic chains after insertion of azithromycin in lipids. The findings provide a molecular insight of the phase merging of DPPC gel in DOPC fluid matrix induced by azithromycin [Berquand, A., Mingeot-Leclercq, M.P., Dufrene, YE, 2004. Real-time imaging of drug-membrane interactions by atomic force microscopy. Biochim. Biophys. Acta 1664, 198-205] and could help to a better understanding of azithromycin-cell interaction. (c) 2006 Elsevier Ireland Ltd. All rights reserved.