TOWARDS THE BIOLOGICAL-CONTROL OF FUNGAL AND BACTERIAL DISEASES OF TOMATO USING ANTAGONISTIC STREPTOMYCES SPP
TOWARDS THE BIOLOGICAL-CONTROL OF FUNGAL AND BACTERIAL DISEASES OF TOMATO USING ANTAGONISTIC STREPTOMYCES SPP
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DOI：
10.1007/bf00016608
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发表时间：
1993-02-01
期刊：
影响因子：
4.9
通讯作者：
ELSABBAGH, SM
中科院分区：
文献类型：
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作者：
ELABYAD, MS;ELSAYED, MA;ELSABBAGH, SM
本研究旨在探索微生物拮抗作用在番茄细菌性、枯萎病、黄萎病、早疫病、细菌性溃烂病防治中的应用潜力。三株链霉菌体外研究表明，80%浓度的普氏链霉菌和尖孢链霉菌的培养滤液对尖孢镰刀菌的孢子萌发、菌丝生长和斑点形成均有明显的抑制作用。番茄、Albo-Atrum和茄链格孢菌(Alternaria Solani)相同浓度的普氏链霉菌和橙色荧光链霉菌的滤液对密歇根棒状杆菌亚种的细菌数量都是有害的。体内研究包括不同的处理：播种前将番茄种子浸泡在拮抗菌的滤液中，播种前7天用拮抗菌接种土壤，播种前用拮抗菌的孢子包衣番茄种子。在播后42天和63天，种衣剂对所有病原菌的控制效果最好。与种衣剂相比，播种前7天土壤接种拮抗菌对番茄病原菌的防治效果较差。浸种处理对相关病害的防治效果最差，同时也表明用拮抗链霉菌进行种衣剂包衣。显著促进了番茄的生长。
This investigation was designed to explore the potential of microbial antagonism in the control of some tomato diseases including bacterial, Fusarium and Verticillium wilts; early blight; bacterial canker. Three Streptomyces spp. were used: S. pulcher, S. canescens and S. citreofluorescens.The in vitro studies showed that an 80% concentration of the culture filtrate of either S. pulcher or S. canescens significantly inhibited spore germination, mycelial growth and spotulation of Fusarium oxysporum f.sp. lycopersici, Verticillium albo-atrum and Alternaria solani. The same concentration of filtrate of either S. pulcher or S. citreofluorescens was detrimental to the bacterial populations of Clavibacter michiganensis subsp. michiganensis and Pseudomonas solanacearum.The in vivo studies involved different treatments: soaking tomato seeds in filtrate of the antagonist prior to sowing, inoculation of the soil with the antagonist 7 days before sowing, and coating of tomato seeds with spores of the antagonist before sowing. The seed-coating treatment was the most effective in controlling all the pathogens at 42 and 63 days after sowing. Soil inoculation with the antagonist 7 days prior to sowing was less effective in controlling the tomato pathogens as compared to seed-coating. The seed-soaking treatment was the least effective in controlling the diseases concerned.The results also revealed that seed-coating with antagonistic Streptomyces spp. significantly improved tomato growth.