Inhibition of rat parotid gland growth response induced by chronic isoproterenol following treatment with quinolone antibiotics.
Inhibition of rat parotid gland growth response induced by chronic isoproterenol following treatment with quinolone antibiotics.
复制标题
喹诺酮类抗生素治疗后慢性异丙肾上腺素诱导的大鼠腮腺生长反应的抑制。
DOI：
10.1007/bf00229745
复制
发表时间：
1996
影响因子：
4.3
通讯作者：
Zelles,T
中科院分区：
文献类型：
--
作者：
Kelentey,B;Kerr,M;Tao,Z;Purushotham,KR;Humphreys-Beher,MG;Zelles,T
虽然抗生素广泛用于牙科和医学治疗，但很少有人关注抗生素对组织再生的潜在毒副作用。在这里，我们研究了喹诺酮类抗生素，培氟沙星（罗纳普朗克）对大鼠腮腺反应慢性异丙肾上腺素治疗的影响。各组大鼠接受注射异丙肾上腺素以诱导腺体生长、盐水（对照）、培氟沙星或异丙肾上腺素和培氟沙星的组合。培氟沙星治疗7天后，腮腺重量显着下降，以及抑制由异丙肾上腺素引起的腺体肿大。在DNA合成速率方面观察到相同的趋势，异丙肾上腺素/哌拉西林处理大鼠中[3 H]-胸苷掺入量降低至单独异丙肾上腺素处理水平的49%。生理盐水处理的动物是异丙肾上腺素处理的大鼠中观察到的[3 H]-胸苷掺入DNA的速率的42%。而异丙肾上腺素治疗增加稳态mRNA水平fos，jun，myc，src，c-erbB-2，rasandtopo Ⅱ，包括培氟沙星与异丙肾上腺素方案阻断这些增加。培氟沙星治疗本身并没有改变腮腺中原癌基因mRNA的水平。培氟沙星治疗组的腺淀粉酶活性降低，而异丙肾上腺素与培氟沙星联合治疗不能使腺淀粉酶水平降低到单独β-激动剂治疗观察到的程度。在急性实验中，培氟沙星显著减少腮腺分泌的唾液量。这些结果表明，喹诺酮类抗生素干扰腮腺的分泌功能，可以抑制细胞增殖和再生。(Mol Cell Biochem165：55-63，1996）
While antibiotics are broadly used in dental and medical therapy, little attention has been directed towards the potential toxic side effects of antibiotics on tissue regeneration. Here we examined the effect of a quinolone antibiotic, pefloxacin (Rhone Poulenc) on rat parotid gland responses to chronic isoproterenol treatment. Groups of rats received injections of isoproterenol to induce glandular growth, saline (controls), pefloxacin, or isoproterenol and pefloxacin in combination. Parotid gland weight decreased significantly after pefloxacin treatment for 7 days as well as inhibiting glandular enlargement provoked by isoproterenol. The same trend was observed for the rates of DNA synthesis, with the incorporation of [3H]-thymidine in isoproterenol/pefloxacin-treated rats reduced to 49% of isoproterenol treatment alone levels. Saline-treated animals were 42% of the rate of [3H]-thymidine incorporation into DNA observed in isoproterenol treated rats. While isoproterenol treatment increased steady-state mRNA levels forfos, jun, myc, src, c-erbB-2,rasandtopoII, inclusion of pefloxacin with the isoproterenol regimen blocked these increases. Pefloxacin treatment by itself did not alter proto-oncogene mRNA levels in the parotid gland. Glandular amylase activity was decreased in the pefloxacin treated group, while the combination of isoproterenol with pefloxacin did not decrease glandular amylase levels to the extent of that observed with β-agonist treatment alone. In acute experiments, pefloxacin significantly decreased the volume of saliva secreted by the parotid gland. These results suggest that quinolone-based antibiotics disturb the secretory function of the parotid gland and can inhibit cell proliferation and regeneration. (Mol Cell Biochem165:55–63, 1996)