Growth and development of tetracycline-resistant Chlamydia suis
Growth and development of tetracycline-resistant Chlamydia suis
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DOI：
10.1128/aac.45.8.2198-2203.2001
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发表时间：
2001-08-01
影响因子：
4.9
通讯作者：
Rockey, DD
中科院分区：
文献类型：
--
作者：
Lenart, J;Andersen, AA;Rockey, DD
四环素(Tet)是治疗人和动物衣原体感染的一线抗生素，Tet耐药衣原体(Tet(R))的出现具有重要的临床意义。最近，从内布拉斯加州生产设施饲养的猪(Sus Scrofa)中分离出几种Tet(R)衣原体菌株。在这里，两个Tet(R)菌株R19和R27的细胞内发育通过使用组织培养和免疫荧光来表征，这些菌株在高达4µg/ml的Tet浓度中生长，而Tet敏感(S)猪株(S45)和人L2变种(LGV-434)在高达0.1µg/ml的Tet中生长。虽然在Tet存在的情况下形成内含体，但许多包含大的异常网状小体(RB)，不能分化为有感染性的基本体。随着Tet浓度的增加，含有典型发育形态的包裹体百分率下降，当Tet浓度为3mug/ml时，100%的包裹体含有异常RBS，但当去除Tet后，异常RBS恢复为典型RBS，并伴随着一个有效的发育周期。此外，连续感染后发现同时含有猪链球菌R19和沙眼衣原体L2的包涵体，表明两个生物学上不同的衣原体菌株都可以在一个包裹体中发育。
Tetracycline (TET) is a front-line antibiotic for the treatment of chlamydial infections in both humans and animals, and the emergence of TET-resistant (Tet(r)) Chlamydia is of significant clinical importance. Recently, several Tet(r) chlamydial strains have been isolated from swine (Sus scrofa) raised in production facilities in Nebraska. Here, the intracellular development of of two Tet(r) strains, R19 and R27, is characterized through the use of tissue culture and immunofluorescence, The strains grow in concentrations of up to 4 mug of TET/ml, while a TET-sensitive (Tet(s)) swine strain (S45) and a strain of the human serovar L2 (LGV-434) grow in up to 0.1 mug of TET/ml. Although inclusions form in the presence of TET, many contain large aberrant reticulate bodies (RBs) that do not differentiate into infectious elementary bodies. The percentage of inclusions containing typical developmental forms decreases with increasing TET concentrations, and at 3 mug of TET/ml 100% of inclusions contain aberrant RBs, However, upon removal of TET the aberrant RBs revert to typical RBs, and a productive developmental cycle ensues. In addition, inclusions were found that contained both C. suis R19 and Chlamydia trachomatis L2 after sequential infection, demonstrating that two biologically distinct chlamydial strains could both develop within a single inclusion.