THE USE OF RADIOACTIVE CHROMIUM-51 AS AN ERYTHROCYTE TAGGING AGENT FOR THE DETERMINATION OF RED CELL SURVIVAL INVIVO
THE USE OF RADIOACTIVE CHROMIUM-51 AS AN ERYTHROCYTE TAGGING AGENT FOR THE DETERMINATION OF RED CELL SURVIVAL INVIVO
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DOI：
10.1172/jci102855
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发表时间：
1953-01-01
影响因子：
15.9
通讯作者：
ROSS, JF
中科院分区：
文献类型：
--
作者：
EBAUGH, FG;EMERSON, CP;ROSS, JF
可用于定量测量临床受试者溶血率和评估储存后红细胞活力的最有用方法之一是基于输注红细胞的存活率。然而，由于迄今为止可用于测量体内红细胞存活的方法的局限性，获得此类数据受到限制。计数供体细胞的差异凝集方法是最常用的程序，但其局限性在于需要大量输血；*供体血液必须不含受血者同样不具有的所有抗原同凝素；接受者的细胞必须含有供体血液中不含有的凝集原 A、B 或 M 或三种凝集原的组合；最后，该方法排除了自体输血的使用，这将消除血源性血清黄疸传播的风险。用放射性铁 (Fe55) 标记供体细胞可以进行短期生存研究 (1-3)，但重新利用溶血的供体细胞释放的放射性铁以及随后将该标记掺入受体红细胞中，就无法将其用于持续时间超过 24 至 48 小时的研究。
One of the most useful methods available for the quantitative measurement of hemolytic rates in clinical subjects and for the evaluation of red cell viability after storage is based on the survival of transfused erythrocytes. Access to such data, however, has been restricted because of limitations in the methods hithertofore available for measuring red cell survival in vivo. The differential agglutination method of count-ing donor cells, the procedure most commonly employed, has limitations in that large transfusions are required;* the donor blood must be devoid of all antigenic isoagglutinins which are not likewise possessed by the recipient; the recipient's cells must contain agglutinogen A or B or M or a com-bination of the three agglutinogens which is not contained in the donor blood; and, finally, this method excludes the use of autotransfusion, which would eliminate the risk of transmission of he-mologous serum jaundice. The labelling of donor cells with radioactive iron (Fe55) permits the conduct of survival studies on a short term basis (1-3) but re-utilization of radioac-tive iron released from hemolyzed donor cells and the subsequent incorporation of this label in the re-cipient's red cells preclude its use in studies ex-tending for periods longer than 24 to 48 hours.