Voltage-dependent K channels in mouse glomus cells are modulated by acetylcholine.
Voltage-dependent K channels in mouse glomus cells are modulated by acetylcholine.
复制标题
小鼠血管球细胞中的电压依赖性 K 通道受乙酰胆碱调节。
DOI：
10.1007/0-387-31311-7_49
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发表时间：
2006
影响因子：
--
通讯作者：
Shirahata,Machiko
中科院分区：
文献类型：
--
作者：
Otsubo,Toshiki;Yamaguchi,Shigeki;Shirahata,Machiko
摘要颈动脉体中存在多种神经递质。这些神经递质负责唤起传入神经末梢的动作电位。它们还通过与血管球细胞上的自身受体结合来改变血管球细胞的功能。我们之前已经证明，ACh 会不同程度地影响猫血管球细胞中的电压依赖性 K (Kv) 电流（Shirahata 等人，2002）。血管球细胞的兴奋性由多种类型的 K 通道调节，包括电压依赖性 (Kv) 通道（Buckler，1999；Shirahata 和 Sham，1999）。 Kv 通道随着膜去极化而被激活，并且在细胞膜复极化中发挥重要作用。多项研究表明，Kv 通道的活性受到神经递质的调节（Brown et al., 1997; Dong and White, 2003; Fukuda et al., 1988; Huang et al., 1993; Shi et al., 1999, 2004），这种类型的修饰对于微调血管球细胞的兴奋性可能很重要。最近的研究表明，DBA/2J 近交系小鼠的颈动脉体表现出与猫血管球细胞的形态相似性（Yamaguchi 等，2003）。这些小鼠的肾小球细胞对ACh作出反应（Yamaguchi等人，2003）并表达Kv通道的4-氨基吡啶敏感和charybdotoxin敏感成分（Yamaguchi等人，2004）。在本研究中，我们研究了 DBA/2J 小鼠血管球细胞中的 Kv 通道是否也被 ACh 修饰。此外，还研究了ACh调节Kv电流的一些潜在机制。
AbstractSeveral neurotransmitters are present in the carotid body. These neurotransmitters are responsible to evoke action potentials in afferent nerve endings. They also modify the function of glomus cells, by binding to autoreceptors on glomus cells. We have previously demonstrated that ACh variably influences voltage-dependent K (Kv) current in cat glomus cells (Shirahata et al., 2002). Excitability of glomus cells are regulated by several types of K channels including voltage-dependent (Kv) channels (Buckler, 1999; Shirahata and Sham, 1999). Kv channels are activated with membrane depolarization, and play an essential role for repolarizing the cell membrane. Several studies have shown that the activity of Kv channels are modulated by neurotransmitters (Brown et al., 1997; Dong and White, 2003; Fukuda et al., 1988; Huang et al., 1993; Shi et al., 1999, 2004), and this type of modification may be important for fine tuning of the excitability of glomus cells. Recent studies have shown that the carotid body of DBA/2J inbred strain of mice demonstrates morphological similarities to cat glomus cells (Yamaguchi et al., 2003). Glomus cells of these mice responds to ACh (Yamaguchi et al., 2003) and express 4-aminopyridine sensitive and charybdotoxin sensitive components of Kv channels (Yamaguchi et al., 2004). In this study, we have investigated whether Kv channels in glomus cells of DBA/2J mice are also modified by ACh. Further, some underlying mechanisms of Kv current modulation by ACh was also investigated.