GALECTIN-7, A HUMAN 14-KDA S-LECTIN, SPECIFICALLY EXPRESSED IN KERATINOCYTES AND SENSITIVE TO RETINOIC ACID
GALECTIN-7, A HUMAN 14-KDA S-LECTIN, SPECIFICALLY EXPRESSED IN KERATINOCYTES AND SENSITIVE TO RETINOIC ACID
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DOI：
10.1006/dbio.1995.1078
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发表时间：
1995-04-01
影响因子：
2.7
通讯作者：
DARMON, M
中科院分区：
文献类型：
--
作者：
MAGNALDO, T;BERNERD, F;DARMON, M
编码S-凝集素家族新成员的cDNA已从人表皮中克隆出来。根据目前公认的命名法，由该序列的136个氨基酸开放阅读框预测的pI 7的14-kDa蛋白质被称为galectin-7。 GST 融合蛋白验证了该凝集素亚家族成员预期的乳糖结合特性。 Galectin-7 在角质形成细胞蛋白提取物的二维凝胶上得到鉴定。差异杂交和原位杂交表明该凝集素在角质形成细胞中特异性表达。它在表皮分化的所有阶段（即基底层和基底上层）表达。它被视黄酸中度抑制，这种行为与对该试剂敏感的其他角质形成细胞标记物的行为形成鲜明对比，这些标记物要么是基底的，被诱导的，要么是基底上的，被抑制的。视黄酸对角质形成细胞细胞类型标记物（例如半乳糖凝集素7）的这种作用更让人想起其在体内的化生作用，而不是其在体外对终末表皮分化的抑制作用。这一解释得到了以下事实的支持：在鸡表皮中，14-kDa S-凝集素在视黄酸诱导的粘液化生过程中受到抑制。 (C) 1995 学术出版社
A cDNA encoding a novel member of the S-lectin family has been cloned from human epidermis, The 14-kDa protein of pI 7 predicted by the 136-amino-acid open reading frame of the sequence was called galectin-7 according to the presently accepted nomenclature. A GST fusion protein authentified the lactose-binding properties expected for a member of this lectin subfamily. Galectin-7 was identified on two-dimensional gels of keratinocyte protein extracts. Differential and in situ hybridizations indicate that this lectin is specifically expressed in keratinocytes. It is expressed at all stages of epidermal differentiation (i.e., in basal and suprabasal layers). It is moderately repressed by retinoic acid, a behavior contrasting with those of other keratinocyte markers sensitive to this agent, which, either basal, are induced, or suprabasal, are repressed. This effect of retinoic acid on a keratinocyte cell type marker such as galectin-7 is more reminiscent of its metaplasiogenic effect in vivo than of its inhibitory effect on terminal epidermal differentiation in vitro. This interpretation is supported by the fact that in chick epidermis a 14-kDa S-lectin is suppressed during retinoic acid-induced mucous metaplasia. (C) 1995 Academic Press, Inc.