Spatial localization of transplanted hemopoietic stem cells: inferences for the localization of stem cell niches
Spatial localization of transplanted hemopoietic stem cells: inferences for the localization of stem cell niches
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DOI：
10.1182/blood.v97.8.2293
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发表时间：
2001-04-15
期刊：
影响因子：
20.3
通讯作者：
Coverdale, JA
中科院分区：
文献类型：
--
作者：
Nilsson, SK;Johnston, HM;Coverdale, JA
在单细胞水平上研究了同基因移植后造血祖细胞亚群的空间分布。用5-(和-6)-羧基荧光素二乙酸酯琥珀酰亚胺酯标记细胞和原位灌流固定的方法确定骨髓中输注造血祖细胞的位置。输注后超过15小时的分析表明，移植的骨髓细胞的空间分布不是一个随机过程。虽然大多数细胞从中央骨髓血管进入骨髓，但随后在骨髓中的定位因其表型而异。候选的“干细胞”表现出选择性的再分布，并在骨内区域显著丰富，而成熟的终末分化和谱系定向的细胞选择性地重新分布在远离骨内区域的地方，主要分布在中央骨髓区域。总而言之，这些数据有力地支持了骨内膜造血干细胞生态位存在的历史证据。(血液，2001；97：2293-2299)(C)2001，由美国血液病学会出版。
The spatial distribution of subpopulations of hemopoietic progenitor cells following syngeneic transplantation was investigated at the single-cell level. The location of infused hemopoietic progenitor cells within the femoral bone marrow of nonablated recipients was determined by 5-(and-6)-carboxyfluorescein diacetate succinimidyl ester labeling of cells and in situ fixation by perfusion. Analysis performed over 15 hours after infusion demonstrated that the spatial distribution of transplanted marrow cells is not a random process. Although the majority of cells enter the bone marrow from the central marrow vessels, the subsequent localization within the bone marrow varied according to their phenotype. Candidate "stem cells" demonstrated selective redistribution and were significantly enriched within the endosteal region, whereas mature terminally differentiated and lineage-committed cells selectively redistributed away from the endosteal region and were predominantly in the central marrow region. Together, these data strongly support historical evidence of the presence of endosteal hemopoietic stem cell niches. (Blood, 2001;97:2293-2299) (C) 2001 by The American Society of Hematology.