Functional Similarity and Dierence among Bra-MIR319 Family in Plant Development
Functional Similarity and Dierence among Bra-MIR319 Family in Plant Development
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作者：
Ziwei Hu;Tingting Liu;Jiashu Cao
miR319 was the first plant miRNA discovered via forward genetic mutation screening..In this study, we found that miR319 family members had similar sequences but dierent expression.patterns in Brassica campestris and Arabidopsis thaliana. RT-PCR analysis revealed that Bra-MIR319a.and Bra-MIR319c had similar expression patterns and were widely expressed in plant development,.whereas Bra-MIR319b could only be detected in stems. The overexpression of each Bra-MIR319.family member in Arabidopsis could inhibit cell division and function in leaf and petal morphogenesis..Bra-miR319a formed a new regulatory relationship after whole genome triplication, and Bra-MIR319a.overexpressing in Arabidopsis led to the degradation of pollen content and aected the formation of.intine, thereby causing pollen abortion. Our results suggest that Bra-MIR319 family members have.functional similarity and dierence in plant development.
