Characterization of diverse hydrocarbon-degrading bacteria isolated from Indonesian seawater
Characterization of diverse hydrocarbon-degrading bacteria isolated from Indonesian seawater
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DOI：
10.1264/jsme2.22.412
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发表时间：
2007-12-01
影响因子：
2.2
通讯作者：
Watanabe, Kazuya
中科院分区：
文献类型：
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作者：
Harwati, Teresia Umi;Kasai, Yuki;Watanabe, Kazuya
从印度尼西亚塞马朗港采集的海水样品划线接种于涂有风化原油的无机培养基平板上，分离得到153株菌株。16 SrRNA基因序列分析鉴定出67个不同的细菌型，分别属于α变形菌门（111株/44个细菌型）、γ变形菌门（8/8）和放线菌门（34/15）。以36个菌型为代表的微生物在纯培养条件下均能转化石油组分。其中许多属于属，但与烃类降解无关。还获得了与已知属无关的菌株。研究结果表明，许多未知的烃降解细菌存在于热带海洋环境中。
Seawater sampled from the Semarang port in Indonesia was streaked onto inorganic-medium plates coated with weathered crude oil, and 153 strains were isolated. Analyses of 16S rRNA gene sequences identified 67 different phylotypes affiliated with Alphaproteobacteria (111 strains/44 phylotypes), Gammaproteobacteria (8/8) and Actinobacteria (34/15). The organisms represented by 36 phylotypes could transform petroleum components in pure cultures. Many of them were affiliated with genera yet unrelated to hydrocarbon degradation. Strains unaffiliated with known genera were also obtained. Results suggest that many as-yet-unknown hydrocarbon-degrading bacteria are present in tropical marine environments.