EFFECTS OF THE PROLINE ANALOG L-THIAZOLIDINE-4-CARBOXYLIC ACID ON PROLINE METABOLISM
EFFECTS OF THE PROLINE ANALOG L-THIAZOLIDINE-4-CARBOXYLIC ACID ON PROLINE METABOLISM
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DOI：
10.1104/pp.74.2.213
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发表时间：
1984-01-01
期刊：
影响因子：
7.4
通讯作者：
STEWART, CR
中科院分区：
文献类型：
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作者：
ELTHON, TE;STEWART, CR
研究了不同的脯氨酸类似物对黄化大麦(Hordeum Vusgare)幼苗线粒体中脯氨酸氧化的影响。在所测试的类似物中，只有L-噻唑烷-4-羧酸(T4C)是有效的抑制剂。T4C(1 MM)对依赖10 mM的Pro摄取的抑制作用平均为67%。T4C也有不同程度的氧化(12.9个纳米原子O2/分钟每毫克蛋白10 mm)。还检测了T4C对其他线粒体底物氧化的影响。T4C对DELTA1-吡咯烷-5-羧酸依赖的氧摄取有轻微的抑制作用(13%)，对苹果酸+丙酮酸的氧化抑制作用更小(6%)，对琥珀酸(+11%)、外源NADH(+19%)和柠檬酸(+20%)的氧化有促进作用。因此，T4C对线粒体的抑制作用是相对专一性的。研究发现，T4C能抑制Pro脱氢酶，但不能抑制Pro向基质的转运。研究了T4C对青大麦离体液中脯氨酸代谢的影响。T4C抑制膨大叶片中的脯氨酸氧化，使叶片中的Pro含量略有增加。在枯萎的叶片(快速合成Pro)中，T4C抑制了Pro的合成，导致叶片中的Pro含量下降。1-吡咯烷-5-羧酸还原酶(合成脯氨酸的最后一种酶)不受T4C的抑制，因此T4C的影响先于合成途径的那一步。T4C对蛋白质中的脯氨酸掺入没有影响。
The effect of various proline analogs on proline oxidation in mitochondria isolated from etiolated barely (Hordeum vulgare) shoots was investigated. Of the analogs tested, only L-thiazolidine-4-carboxylic acid (T4C) was an effective inhibitor. T4C (1 mM) inhibited proline (10 mM) -dependent uptake an average of 67%. T4C was also oxidized to some degree (12.9 nanoatoms O2/min per mg protein for 10 mM.). The effect of T4C on the oxidation of other mitochondrial substrates was also tested. T4C inhibited .DELTA.1-pyrrolidine-5-carboxylic acid-dependent O2 uptake slightly (13%), the oxidation of malate plus pyruvate even less (6%), and stimulated the oxidation of succinate (+11%), exogenous NADH (+19%), and citrate (+20%). Thus, inhibition by T4C in mitochondria is relatively specific to proline oxidation. T4C was found to inhibit proline dehydrogenase and not the transport of proline into the matrix. The effect of T4C on proline metabolism in detached green barley levels was investigated. T4C inhibited proline oxidation in turgid leaves, increasing the proline content of these leaves slightly. In wilted leaves (that are synthesizing proline rapidly), T4C inhibited proline synthesis, which resulted in a decrease in the proline content of the leaves. .DELTA.1-pyrrolidine-5-carboxylic acid reductase (the last enzyme in proline synthesis) was not inhibited by T4C, and thus T4C influence is prior to that step of the synthetic pathway. T4C had no influence on the incorporation of proline into protein.