The new mutation L321F in Candida albicans ERG11 gene may be associated with fluconazole resistance
The new mutation L321F in Candida albicans ERG11 gene may be associated with fluconazole resistance
复制标题
DOI：
10.1016/j.riam.2013.01.001
复制
发表时间：
2013-07-31
影响因子：
1.9
通讯作者：
del Negro, Gilda M. B.
中科院分区：
文献类型：
--
作者：
Carvalho, Vagner Oliveira;Okay, Thelma S.;del Negro, Gilda M. B.
背景：多年来，氟康唑一直是治疗念珠菌感染的常用药物。然而，这种抗真菌治疗的滥用有利于耐药分离株的出现。ERG 11基因突变是念珠菌耐药的主要机制之一，本研究对白色念珠菌、光滑念珠菌和热带念珠菌的ERG 11基因错义突变进行了研究。白色念珠菌5株。glabrata和6个C.对氟康唑耐药和敏感。ERG11基因通过PCR扩增与每个念珠菌物种的特异性引物和分析通过自动sequencing.Results：我们确定了14个不同的错义突变，其中5个没有被描述过。其中，在一株对氟康唑耐药的念珠菌中发现了一个新的突变L321F。结论：白念珠菌L321F突变可能与ERG11p的三维结构有关。白念珠菌ERG 11基因可能与氟康唑耐药相关。(c)2012年《伊比利亚美洲微生物学杂志》。出版社：Elsevier Espana，S.L. All rights reserved.
Background: For many years fluconazole has been commonly used to treat Candida infections. However, the indiscriminate use of this antimycotic therapy has favored the emergence of resistant isolates. Mutations in the ERG11 gene have been described as one of the primary mechanisms of resistance in Candida species.Aims: In this study we investigated missense mutations in ERG11 genes of Candida albicans, Candida glabrata and Candida tropicalis isolates previously evaluated by susceptibility testing to fluconazole.Methods: Screening for these mutations was performed on 19 Candida clinical isolates (eight C. albicans, five C. glabrata and six C. tropicalis) resistant and susceptible to fluconazole. The ERG11 gene was amplified by PCR with specific primers for each Candida species and analyzed by automated sequencing.Results: We identified 14 different missense mutations, five of which had not been described previously. Among them, a new mutation L321F was identified in a fluconazole resistant C. albicans isolate and it was analyzed by a theoretical three-dimensional structure of the ERG11p.Conclusion: The L321F mutation in C. albicans ERG11 gene may be associated with fluconazole resistance.(c) 2012 Revista lberoamericana de Micologia. Published by Elsevier Espana, S.L. All rights reserved.