Effect of UV-B radiation on photosystem II functions in Antarctic and mesophilic strains of a green alga Chlorella vulgaris and a cyanobacteritum Synechocystis salina
Effect of UV-B radiation on photosystem II functions in Antarctic and mesophilic strains of a green alga Chlorella vulgaris and a cyanobacteritum Synechocystis salina
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DOI：
10.1007/s40502-014-0084-9
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发表时间：
2014-06-01
期刊：
影响因子：
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通讯作者：
Misra, Amarendra N.
中科院分区：
文献类型：
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作者：
Apostolova, Emilia L.;Pouneva, Irina;Misra, Amarendra N.
从南极和中温环境中分离的绿色小球藻和集胞藻细胞，在连续可见光下分批培养，用于评估UV-B辐射对光系统II（PSII）的影响。通过脉冲幅度调制叶绿素荧光和氧释放（用氧速率电极测量）估计UV诱导的功能活性变化。这些数据揭示了一个相对较强的UV-B诱导的抑制放氧相比，初级光化学的PSII在绿色藻类和蓝藻。氧释放的抑制是功能活性PSII中心的数量减少的结果。对活性反应中心的修饰也表现为对快反应中心的修饰作用大于慢反应中心。另一方面，蓝藻的PSII活性比绿色藻类更容易受到UV-B辐射的影响。同样地，S.桑拿更容易受到UV-B辐射比南极分离物。
The green alga Chlorella vulgaris and the cyanobacterium Synechocystis sauna cells isolated from Antarctic and mesophific environments, grown in batch cultures under continuous visible light, were used to assess the effect of the UV-B radiation on the photosystem II (PSII). UV-induced changes in its functional activity were estimated by Pulse Amplitude Modulated chlorophyll fluorescence and oxygen evolution (measured with oxygen rate electrode). The data reveal a relatively stronger UV-B-induced inhibition of oxygen evolution in comparison to that of the primary photochemistry of the PSII in both green algae and cyanobacteria. The inhibition of oxygen evolution was a result of the decrease in the number of functionally active PSII centers. The modification of active reaction centers was also recorded through the relatively more effect on fast operating PSII centers than that of the slow operating PSII centers. On the other hand, the PSII activity of cyanobacteria was more vulnerable to UV-B radiation than that of green algae. Likewise, the mesophific strain of S. sauna was more susceptible to UV-B radiation than the Antarctic isolates.