HYPOXIC CONTRACTION OF CANINE CORONARY-ARTERIES - ROLE OF ENDOTHELIUM AND CGMP
HYPOXIC CONTRACTION OF CANINE CORONARY-ARTERIES - ROLE OF ENDOTHELIUM AND CGMP
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DOI：
10.1152/ajpheart.1991.261.6.h1769
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发表时间：
1991-12-01
影响因子：
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通讯作者：
VANHOUTTE, PM
中科院分区：
文献类型：
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作者：
GRASER, T;VANHOUTTE, PM
研究了严重缺氧对静息或收缩（前列腺素F2-α）犬冠状动脉环（有和无内皮）的影响。缺氧引起了最初的短暂放松，然后是持续的收缩。静止环中的缺氧收缩在有内皮和无内皮的环中是相当的。对前列腺素F2-α的收缩的促进作用在具有内皮的环中更明显。通过增加前列腺素F2-α的收缩来增加收缩水平，增强了仅具有内皮的环中的缺氧收缩。亚甲蓝、LY 83583和硝基-L-精氨酸可逆转缺氧对收缩血管环的收缩促进作用，而M&B 22948可增强缺氧对收缩血管环的舒张促进作用。SIN-1、硝酸甘油和二丁酰鸟苷3 '，5'-环磷酸（cGMP）揭示了无内皮环中的缺氧易化作用。使用异丙肾上腺素时未观察到这一点。cGMP水平的测量显示，在控制氧合下，与无内皮的环相比，有内皮的环中cGMP水平升高。缺氧时，这种差异消失，这是由于有内皮的血管中cGMP含量减少。结果表明，在收缩的犬冠状动脉中，血管平滑肌中cGMP水平的适度增加是缺氧诱导易化发生的先决条件。
The effect of severe hypoxia in quiescent or contracted (prostaglandin F2-alpha) canine coronary artery rings with and without endothelium was studied. Hypoxia induced an initial transient relaxation followed by a sustained contraction. The hypoxic contraction in quiescent rings was comparable in rings with and without endothelium. The facilitation of the contraction to prostaglandin F2-alpha was more pronounced in rings with endothelium. Increasing the level of contractions by augmenting the contraction of prostaglandin F2-alpha potentiated the hypoxic contraction in rings with endothelium only. Methylene blue, LY 83583, and nitro-L-arginine reversed the hypoxic facilitation in contracted rings into relaxation, whereas M&B 22948 augmented it. In quiescent coronary preparations, methylene blue reversed the hypoxic contraction into relaxation in preparations with and without endothelium, whereas nitro-L-arginine had the same effect in vessels with endothelium only. SIN-1, nitroglycerin, and dibutyryl guanosine 3',5'-cyclic monophosphate (cGMP) unmasked hypoxic facilitation in rings without endothelium. This was not observed with isoproterenol. The measurement of the level of cGMP revealed an increased level in rings with endothelium compared with those without endothelium under control oxygenation. This difference disappeared during hypoxia due to a decrease of cGMP content in vessels with endothelium. The results suggest that a moderate increase of the cGMP level in vascular smooth muscle is a prerequisite for the occurrence of hypoxia-induced facilitation in contracted canine coronary arteries.