Noninvasive evaluation of intrarenal oxygenation with BOLD MRI
Noninvasive evaluation of intrarenal oxygenation with BOLD MRI
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DOI：
10.1161/01.cir.94.12.3271
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发表时间：
1996-12-15
期刊：
影响因子：
37.8
通讯作者：
Epstein, FH
中科院分区：
文献类型：
--
作者：
Prasad, PV;Edelman, RR;Epstein, FH
背景哺乳动物肾脏髓质中毛细血管血流的逆流排列在肾皮质和乳头尖端之间产生氧张力梯度，导致肾髓质内的相对缺氧状态。由于缺乏一种无创性技术来估计肾脏不同区域的肾内氧合，因此对髓质缺氧的病理生理学意义的探索受到阻碍。在本研究中，我们证明了这种方法的可行性的基础上的血氧水平依赖（BOLD）MRI，它允许连续测量人类在响应于各种生理/药理刺激的健康和diseases.Methods和结果BOLD MRI测量获得健康的年轻人受试者（n=7），并研究了诱导利尿的三种不同药理学/生理学操作的效果。测量自旋-自旋弛豫速率R（2）*，其与血液中脱氧血红蛋白的量直接相关，进而与组织PO 2直接相关。呋塞米而非乙酰唑胺（各6例）可增加延髓氧合（Δ R（2）*=7.62 Hz; P
Background The countercurrent arrangement of capillary blood flow in the medulla of mammalian kidneys generates a gradient of oxygen tension between the renal cortex and the papillary tip that results in a state of relative hypoxia within the renal medulla. Exploration of the pathophysiological implications of medullary hypoxia has been hampered by the absence of a noninvasive technique to estimate intrarenal oxygenation in different zones of the kidney. In the present study, we demonstrate the feasibility of such a method on the basis of blood oxygenation level-dependent (BOLD) MRI, which allows sequential measurements in humans in response to a variety of physiological/pharmacological stimuli in health and disease.Methods and Results BOLD MRI measurements were obtained in healthy young human subjects (n=7), and the effects of three different pharmacological/physiological maneuvers that induce diuresis were studied. Spin-spin relaxation rate, R(2)*, was measured, which is directly related to the amount of deoxyhemoglobin in blood and in turn to tissue PO2. Furosemide but not acetazolamide (n=6 each) increased medullary oxygenation (Delta R(2)*=7.62 Hz; P