Therapeutic effects of quinine in a mouse model of atopic dermatitis
Therapeutic effects of quinine in a mouse model of atopic dermatitis
复制标题
奎宁对特应性皮炎小鼠模型的治疗作用。
DOI：
10.3892/mmr.2021.11952
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发表时间：
2021-05-01
影响因子：
3.4
通讯作者：
Wang, Li
中科院分区：
文献类型：
--
作者：
Zhang, Qian;Jiang, Hongjing;Wang, Li
特应性皮炎（AD）是一种严重影响生活质量的慢性炎症性皮肤病。奎宁是一种苦味受体激动剂，具有抗疟疾作用。本研究的目的是检查奎宁在AD样小鼠中的治疗作用。用2，4-二硝基氯苯诱发AD，用奎宁10 mg/kg处理1、4和7 d。将60只BALB/c小鼠随机分为健康、AD样、AD样+奎宁和健康+奎宁4组，每组各1、4和7天。从所有小鼠中提取血液并进行ELISA以检测免疫球蛋白E（IgE）水平。取小鼠背部皮损制备H& E染色组织切片，观察病理变化。ELISA法检测细胞因子水平，免疫印迹法和免疫组化法检测丝聚蛋白（FLG）和激肽释放酶-7（KLK-7）蛋白的表达。通过逆转录-定量PCR分析IKK α和NF-κ B mRNA。奎宁改善AD样小鼠的皮肤损伤，降低血液中IgE的表达，抑制IKK α和NF-κ B的表达，减少细胞因子分泌，减少KLK 7的表达，减少抓挠频率，增加FLG表达并修复皮肤屏障。以上结果提示奎宁对AD样小鼠有治疗作用。
Atopic dermatitis (AD) is a chronic inflammatory skin disease that seriously affects quality of life. Quinine is a bitter taste receptor agonist that exhibits antimalarial effects. The aim of the present study was to examine the therapeutic effects of quinine in AD-like mice. AD was induced with 2,4-dinitrochlorobenzene, and the mice were treated with 10 mg/kg quinine for 1, 4 and 7 days. A total of 60 BALB/c mice were divided into the following groups: Healthy, AD-like, AD-like + quinine and healthy + quinine, with 1, 4 and 7 days groups for each treatment. Blood was extracted from all mice and ELISA was performed to detect immunoglobulin E (IgE) levels. H&E-stained tissue sections were prepared from skin lesions on the backs of the mice and pathological changes were observed. Cytokines were detected via ELISA, and the filaggrin (FLG) and kallikrein-7 (KLK7) proteins were detected via western blotting and immunohistochemistry. IKK alpha and NF-kappa B mRNA were analyzed via reverse transcription-quantitative PCR. Quinine ameliorated skin damage in the AD-like mice, reduced IgE expression in the blood, inhibited expression of IKK alpha and NF-kappa B, reduced cytokine secretion, reduced KLK7 expression, reduced scratching frequency, increased FLG expression and repaired the skin barrier. These results suggested that quinine exhibited therapeutic effects in AD-like mice.