Interleukin-2 receptor beta-chain (p70-75) expressed on leukemic cells from adult T cell leukemia patients.
Interleukin-2 receptor beta-chain (p70-75) expressed on leukemic cells from adult T cell leukemia patients.
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DOI：
10.1111/j.1349-7006.1990.tb02665.x
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发表时间：
1990-09
期刊：
影响因子：
--
通讯作者：
Araki K
中科院分区：
文献类型：
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作者：
Kodaka T;Uchiyama T;Ishikawa T;Kamio M;Onishi R;Itoh K;Hori T;Uchino H;Tsudo M;Araki K
为了确定白细胞介素2受体（IL-2R）β链（p70 - 75）是否在成人T细胞白血病（ATL）患者的白血病细胞上表达以及α链（p55，Tac），我们使用新开发的抗IL-2R β链抗体进行了放射性标记的IL-2（IL-2）结合试验、放射性标记的IL-2的化学交联和流式细胞术分析。结果显示，我们检查的所有12名ATL患者的白血病细胞均表达IL-2R β-链和α-链，而健康志愿者未刺激的外周血CD4（+）T细胞上没有可检测到的β-链表达。Southern杂交分析表明，这种异常表达不是由IL-2R β链基因结构改变引起的。尽管所有ATL患者的白血病细胞都表达高亲和力IL-2R，但只有25%的ATL患者的白血病细胞对IL-2产生反应而增殖。这些结果显示ATL患者白血病细胞（ATL细胞）上IL-2R β-链的异常表达，表明HTLV-I感染与异常β-链表达以及α-链表达之间存在密切关联。
To determine whether the interleukin‐2 receptor (IL‐2R) β‐chain (p70–75) is expressed on lenkemic cells from patients with adult T cell leukemia (ATL) as well as α‐chain (p55, Tac), we performed radiolabeled interleukin‐2 (IL‐2) binding assay, chemical crosslinking of radiolabeled IL‐2 and flow cytometric analysis using a newly‐developed anti‐IL‐2R β‐chain antibody. The results showed that leukemic cells from all the 12 ATL patients we examined expressed the IL‐2R β‐chain together with the α‐chain, whereas there was no detectable β‐chain expression on unstimulated peripheral blood CD4(+) T cells from healthy volunteers. Southern blot analysis revealed that this abnormal expression was not caused by the structural change of IL‐2R β‐chain gene. Though leukemic cells from all ATL patients examined expressed high‐affinity IL‐2Rs, leukemic cells from only 25% of all ATL patients proliferated in response to IL‐2. These results showing abnormal expression of IL‐2R β‐chain on leukemic cells from ATL patients (ATL cells) suggest a close association between HTLV‐I infection and abnormal β‐chain expression as well as α‐chain expression.