Myelin basic protein component C1 in increasing concentrations can elicit fusion, aggregation, and fragmentation of myelin-like membranes
Myelin basic protein component C1 in increasing concentrations can elicit fusion, aggregation, and fragmentation of myelin-like membranes
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DOI：
10.1078/s0171-9335(04)70036-9
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发表时间：
2000-05-01
影响因子：
6.6
通讯作者：
Harauz, G
中科院分区：
文献类型：
--
作者：
Mac Millan, SV;Ishiyama, N;Harauz, G
髓鞘碱性蛋白（MBP）被认为在髓鞘的形成和维持中具有主要作用。许多研究使用简单脂质组成的人工囊泡系统，通常小的尺寸，已经表明，MBP可以引起囊泡融合，聚集，甚至在不同的条件下破碎。在这里，我们已经研究了增加浓度的牛MBP电荷异构体C1（MBP/C1）的影响大单层囊泡（LUVs）组成的磷脂酰胆碱和磷脂酰丝氨酸（92：8摩尔比），或与脂质组合物类似的髓鞘膜在体内（Cyt-LUVs），使用吸光度法，荧光共振能量转移，动态光散射和透射电子显微镜，我们已经表明，囊泡聚集和一些囊泡融合发生后，添加MBP/C1，并作为蛋白质-脂质的摩尔比增加。在非常高的蛋白质浓度下观察到Cyt-LUV片段化。这些结果表明，囊泡的融合，聚集和破碎的现象都可以在一个体外系统中观察到，但依赖于脂质的组成和蛋白质和脂质的相对比例。
Myelin basic protein (MBP) is considered to have a primary role in the formation and maintenance of the myelin sheath. Many studies using artificial vesicle systems of simple Lipid composition, and generally small size, have shown that MBP can elicit vesicle fusion, aggregation, or even fragmentation under different conditions. Here, we have studied the effects of increasing concentrations of bovine MBP charge isomer C1 (MBP/C1) on large unilamellar vesicles (LUVs) composed of phosphatidylcholine and phosphatidylserine (92:8 molar ratio), or with a lipid composition similar to that of the myelin membrane in vivo (Cyt-LUVs), Using absorbance spectrophotometry, fluorescence resonance energy transfer, dynamic light scattering and transmission electron microscopy, we have shown that vesicle aggregation and some vesicle fusion occurred upon addition of MBP/C1, and as the molar protein-lipid ratio increased. Fragmentation of Cyt-LUVs was observed at very high protein concentrations. These results showed that the phenomena of vesicle fusion, aggregation, and fragmentation can all be observed in one in vitro system, but were dependent on lipid composition and on the relative proportions of protein and lipid.