Polysaccharide from Radix Codonopsis has beneficial effects on the maintenance of T-cell balance in mice
Polysaccharide from Radix Codonopsis has beneficial effects on the maintenance of T-cell balance in mice
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党参多糖对维持小鼠 T 细胞平衡具有有益作用。
DOI：
10.1016/j.biopha.2019.108682
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发表时间：
2019-04-01
影响因子：
7.5
通讯作者：
Zhou, Lian
中科院分区：
文献类型：
--
作者：
Deng, Xiangliang;Fu, Yajun;Zhou, Lian
由于免疫平衡而产生的免疫力有助于疾病的预防和治疗。党参多糖是从中药党参的根中分离得到的一种多糖。以往的研究表明，RCP具有免疫调节活性，然而，RCP对免疫，特别是免疫平衡的影响，在很大程度上仍然是未知的。在本研究中，我们研究了RCP对小鼠T细胞平衡的影响。用或不用RCP灌胃预处理小鼠15天，并在第10-15天注射氢化可的松以干扰免疫系统。取脾脏和胸腺称重，计算免疫器官指数。采用流式细胞仪检测外周血中CD 4（+）T细胞、CD 8（+）T细胞、Th 1细胞、Th 2细胞、调节性T细胞和Th 17细胞的百分比。通过酶联免疫吸附测定（ELISA）试剂盒测定血清中促炎和抗炎细胞因子TNF-α、IL-1 β和IL-10。结果表明，RCP预处理可以维持氢化可的松处理小鼠的CD 8（+）T细胞、Tregs、Th 17细胞、TNF-α、IL-1 β和IL-10的动态平衡。此外，RCP预处理可维持CD 4（+）/CD 8（+）T细胞、Th 1/Th 2细胞、Th 2/Th 17细胞、IL-10/TNF-α和IL-10/IL-1 β的免疫平衡。综上所述，RCP预处理对维持小鼠T细胞平衡对抗氢化可的松紊乱具有有益作用，并有可能开发成新型功能性食品。
Immunity due to immune balance contributes to disease prevention and treatment. Radix Codonopsis polysaccharide (RCP) is isolated from the root of the Chinese herb Codonopsis pilosula. Previous studies have indicated that RCP has immunomodulatory activities; however, the effects of RCP on immunity, especially immune balance, are still largely unknown. In the present study, we investigated the effects of RCP on T-cell balance in mice. The mice were pretreated intragastrically with or without RCP for 15 days and injected with hydrocortisone on days 10-15 to disturb the immune system. The spleen and thymus were weighed and used to calculate immune organ indexes. The percentages of CD4(+) T cells, CD8(+) T cells, Th1 cells, Th2 cells, regulatory T cells (Tregs) and Th17 cells in peripheral blood were assayed by flow cytometry. Pro-inflammatory and anti-inflammatory cytokines, TNF-alpha, IL-1 beta and IL-10, in serum were determined by enzyme-linked immunosorbent assay (ELISA) kits. The results showed that RCP pretreatment could maintain the homeostasis of CD8(+) T cells, Tregs, Th17 cells, TNF-alpha, IL-1 beta and IL-10 in hydrocortisone-treated mice. Furthermore, RCP pretreatment maintained the immune balance of CD4(+)/CD8(+) T cells, Th1/Th2 cells, Tregs/Th17 cells, IL-10/TNF-alpha and IL10/IL-1 beta. Taken together, RCP pretreatment had beneficial effects on the maintenance of T-cell balance against hydrocortisone disturbance in mice and potential to be developed into novel functional food.