Influence of albumin on the hepatic uptake of unconjugated bilirubin.
Influence of albumin on the hepatic uptake of unconjugated bilirubin.
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白蛋白对肝脏摄取未结合胆红素的影响。
DOI：
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发表时间：
1973
期刊：
影响因子：
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通讯作者：
Nathaniel I. Berlin
中科院分区：
文献类型：
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作者：
Joseph R. Bloomer;Paul D. Berk;J. Vergalla;Nathaniel I. Berlin
1. 6名成人受试者同时静脉注射两种化合物后，[3h]胆红素的初始分布体积与131 i标记白蛋白的初始分布体积相当。然而，血浆中[3h]胆红素的初始损失速度比131 i标记的白蛋白快7到30倍。2. Gunn大鼠肝片对[3h]胆红素的体外摄取速度比白蛋白相关运动快3倍。3. [14c]胆红素没有从白蛋白转移到大鼠肝细胞质的有机阴离子结合蛋白。相反，白蛋白显示从这些蛋白中剥离[14c]胆红素。4. 当与[14c]胆红素结合的白蛋白与抗白蛋白抗体沉淀时，[14c]胆红素在上清液中完全恢复，这表明如果白蛋白与另一大分子相互作用，未结合的胆红素可能很容易从白蛋白中分离出来。5. 这些结果表明，未结合的胆红素在被肝细胞摄取之前从白蛋白中分离出来，而不是整个白蛋白-胆红素复合物进入肝细胞。数据表明摄取机制可能需要质膜的参与。
1. The initial distribution volumes of [ 3 H]bilirubin in six adult subjects were equivalent to those of 131 I-labelled albumin following simultaneous intravenous injection of both compounds. However, the initial rate of loss of [ 3 H]bilirubin from plasma was seven to thirty times faster than that of 131 I-labelled albumin. 2. In vitro uptake of [ 3 H]bilirubin by Gunn rat liver slices occurred at a rate three times faster than could be accounted for by movement linked with albumin. 3. There was no in vitro transfer of [ 14 C]bilirubin from albumin to the organic anion-binding proteins of rat liver cytosol. Instead, albumin was shown to strip [ 14 C]bilirubin from these proteins. 4. When albumin, to which [ 14 C]bilirubin had been bound, was precipitated with anti-albumin antibody, [ 14 C]bilirubin was recovered completely in the supernatant, indicating that unconjugated bilirubin may be readily separated from albumin if albumin interacts with another macromolecule. 5. These results indicated that unconjugated bilirubin is separated from albumin prior to its uptake by the hepatic cell, rather than the entire albumin-bilirubin complex entering the hepatocyte. The data suggest that the uptake mechanism probably requires the participation of the plasma membrane.