DEVELOPMENT OF AN RFLP MAP IN DIPLOID ALFALFA
DEVELOPMENT OF AN RFLP MAP IN DIPLOID ALFALFA
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DOI：
10.1007/bf00222097
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发表时间：
1993-04-01
影响因子：
5.4
通讯作者：
KOCHERT, G
中科院分区：
文献类型：
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作者：
BRUMMER, EC;BOUTON, JH;KOCHERT, G
我们在二倍体苜蓿（Medicago sativa L.）中开发了一种限制性片段长度多态性（RFLP）连锁图谱，用作苜蓿改良计划的工具。从W2xiso群体（紫花苜蓿亚种sativa）的一株植物与美国农业部PI440501（紫花苜蓿亚种coerulea）的一株植物杂交产生了一个包含86个个体的F2作图群体。当前的图谱包含108个cDNA标记，覆盖467.5厘摩。图谱长度较短可能是由于该杂交中的重组率较低。即使克隆之间的距离可能改变，标记顺序在其他群体中可能保持不变。大约50%的作图位点显示出分离扭曲，主要是偏向过量的杂合子。这是支持最大杂合子理论的间接证据，该理论指出相对活力取决于使具有多个等位基因的位点数量最大化。讨论了该图谱在四倍体群体中的应用。
We have developed a restriction fragment length polymorphism (RFLP) linkage map in diploid alfalfa (Medicago sativa L.) to be used as a tool in alfalfa improvement programs. An F2 mapping population of 86 individuals was produced from a cross between a plant of the W2xiso population (M. sativa ssp. sativa) and a plant from USDA PI440501 (M. sativa ssp. coerulea). The current map contains 108 cDNA markers covering 467.5 centimorgans. The short length of the map is probably due to low recombination in this cross. Marker order may be maintained in other populations even though the distance between clones may change. About 50% of the mapped loci showed segregation distortion, mostly toward excess heterozygotes. This is circumstantial evidence supporting the maximum heterozygote theory which states that relative vigor is dependent on maximizing the number of loci with multiple alleles. The application of the map to tetraploid populations is discussed.