Retinol palmitate prevents ischemia-induced cell changes in hippocampal neurons through the Notch1 signaling pathway in mice
Retinol palmitate prevents ischemia-induced cell changes in hippocampal neurons through the Notch1 signaling pathway in mice
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DOI：
10.1016/j.expneurol.2013.04.014
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发表时间：
2013-09
影响因子：
5.3
通讯作者：
J. Shimada;J. Taniguchi;M. Mori;Yasunori Sato;H. Takuwa;Hiroshi Ito;S. Kuwabara
中科院分区：
文献类型：
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作者：
J. Shimada;J. Taniguchi;M. Mori;Yasunori Sato;H. Takuwa;Hiroshi Ito;S. Kuwabara
视黄醇棕榈酸酯是维生素A的类似物，在神经系统中发挥多种作用，包括神经分化、轴突生长和神经模式形成，也是一种抗氧化剂，因此对脑缺血具有潜在的神经保护作用。本研究旨在探讨视黄醇棕榈酸酯对小鼠双侧颈总动脉闭塞(BCCAO)模型缺血性脑损伤的保护作用。大鼠脑缺血20min后，海马区尤其是CA1区神经元形态发生显著变化，星形胶质细胞反应性增殖，并伴有Notch1表达增加。缺血前腹腔注射视黄醇棕榈酸酯可减少Notch1表达的缺血神经元，1.2 mg/kg组和12 mg/kg组差异均有统计学意义。这些结果表明，视黄醇棕榈酸酯通过Notch1的表达来预防脑缺血所致的神经元损伤，Notch1信号可能参与了神经保护机制。视黄醇棕榈酸酯可能是治疗人类脑梗塞的一种选择。
Retinol palmitate, an analog of vitamin A, plays multiple roles in the nervous system, including neural differentiation, axon outgrowth, and neural patterning, and is also an antioxidative agent and thereby potential neuroprotectant for brain ischemia. The present study aimed at investigating the protective effects of retinol palmitate against ischemia-induced brain injury in a bilateral common carotid artery occlusion (BCCAO) model in mice. Ischemia induced by 20-min BCCAO resulted in significant neuronal morphological changes and reactive astrocyte proliferation in the hippocampus, particularly in the CA1 region, and these changes were accompanied by increased Notch1 expression. Intraperitoneal retinol palmitate administration before ischemia reduced ischemic neurons with Notch1 expression; the differences were statistically significant in both the 1.2 mg/kg group and 12 mg/kg group. These results show that retinol palmitate prevents brain ischemia-induced neuronal injury with Notch1 expression and that Notch1 signaling could be involved in the neuroprotective mechanism. Retinol palmitate could be a treatment option for human brain infarction.