GENOMIC STRUCTURE AND ANALYSIS OF PROMOTER SEQUENCE OF A MOUSE MU-OPIOID RECEPTOR GENE
GENOMIC STRUCTURE AND ANALYSIS OF PROMOTER SEQUENCE OF A MOUSE MU-OPIOID RECEPTOR GENE
复制标题
DOI：
10.1073/pnas.91.19.9081
复制
发表时间：
1994-09-13
影响因子：
11.1
通讯作者：
LOH, HH
中科院分区：
文献类型：
--
作者：
MIN, BH;AUGUSTIN, LB;LOH, HH
我们分离了小鼠μ阿片受体基因组克隆（称为莫尔），其含有对应于大鼠莫尔-1 cDNA的完整氨基酸编码序列，包括另外的5'侧翼序列。小鼠莫尔基因长度>53 kb，编码序列被三个内含子分开，外显子连接点位于密码子95和213之间以及密码子386和387之间。第一个内含子>26 kb，第二个内含子为0.8 kb，第三个内含子>12 kb。观察到多个转录起始位点，通过cDNA末端的5'快速扩增和位于翻译起始密码子上游291和268 bp之间的RNA酶保护证实了四个主要位点。5'侧翼序列与转录因子数据库的比较揭示了受cAMP影响的转录因子的推定顺式作用调节元件，以及参与葡萄糖和盐皮质激素、细胞因子和免疫细胞特异性因子的作用的那些。
We have isolated mouse mu opioid receptor genomic clones (termed MOR) containing the entire amino acid coding sequence corresponding to rat MOR-1 cDNA, including additional 5' flanking sequence. The mouse MOR gene is >53 kb long, and the coding sequence is divided by three introns, with exon junctions in codons 95 and 213 and between codons 386 and 387. The first intron is >26 kb, the second is 0.8 kb, and the third is >12 kb. Multiple transcription initiation sites were observed, with four major sites confirmed by 5' rapid amplification of cDNA ends and RNase protection located between 291 and 268 bp upstream of the translation start codon. Comparison of the 5' flanking sequence with a transcription factor database revealed putative cis-acting regulatory elements for transcription factors affected by cAMP, as well as those involved in the action of gluco- and mineralocorticoids, cytokines, and immune cell specific factors.