High production of egc2-related staphylococcal enterotoxins caused a food poisoning outbreak
High production of egc2-related staphylococcal enterotoxins caused a food poisoning outbreak
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大量产生egc2相关葡萄球菌肠毒素引发食物中毒事件
DOI：
10.1016/j.ijfoodmicro.2021.109366
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发表时间：
2021
影响因子：
5.4
通讯作者：
Hu Dong-Liang
中科院分区：
文献类型：
--
作者：
Umeda Kaoru;Ono Hisaya K.;Wada Takayuki;Motooka Daisuke;Nakamura Shota;Nakamura Hiromi;Hu Dong-Liang
食品中产肠毒素金黄色葡萄球菌产生的葡萄球菌肠毒素可引起葡萄球菌食物中毒。我们最近报告了一起由沙门氏菌引起的食源性疫情。与其他研究小组合作，发现了与肠毒素基因簇(EGC)2相关的新的SE/SE样(SEL)基因(seg、sei、sem、sen、seo和selu)。然而，SES产生的致病性仍不清楚。因此，我们在这里研究了与DECC2相关的SES的生产。在食源性暴发期间，金黄色葡萄球菌和剩余食品使用了一种适用于定量SES的夹心酶联免疫吸附分析方法。金黄色葡萄球菌产生明显高水平的egc2相关SES，而剩余食品“寿司”含有超过导致食物中毒症状的毒素剂量的SES。对一个有代表性的分离株进行了全基因组测序。该分离株与已报道的ST45菌株同源，特别是其独特的基因组岛νSaβ结构主要由egc2组成。目前的研究表明，与第二类相关的SE是食物中毒的致病因素，因为在实际的食源性暴发中，SE的产量很高。
Staphylococcal enterotoxins (SEs) produced by enterotoxigenic Staphylococcus aureus in food cause staphylococcal food poisoning. We recently reported a foodborne outbreak due toS. aureusharboring new SE/SE-like (SEl) genes (seg,sei,sem,sen,seo, andselu) related to enterotoxin gene cluster (egc)2as with other research groups. However, the pathogenicity of SEs production remains unclear. Therefore, we herein investigatedegc2-related SEs production fromS. aureusisolates and leftover food items during a foodborne outbreak using a sandwich enzyme-linked immunosorbent assay suitable for the quantification of SEs.S. aureusisolates produced markedly high levels ofegc2-related SEs, and the leftover food item “Sushi” contained SEs over the toxin dose that causes food poisoning symptoms. A representative isolate was subjected to whole-genome sequencing. The isolate was homologous with previously reported ST45 strains, particularly the unique genomic island νSaβ structure mostly consisting ofegc2. The present study indicates thategc2-related SEs are food poisoning causative agents based on high SE production levels within an actual foodborne outbreak.