Complete amino acid sequence of the light chain of human blood coagulation factor X: evidence for identification of residue 63 as beta-hydroxyaspartic acid.
Complete amino acid sequence of the light chain of human blood coagulation factor X: evidence for identification of residue 63 as beta-hydroxyaspartic acid.
复制标题
人凝血因子 X 轻链的完整氨基酸序列：残基 63 鉴定为 β-羟基天冬氨酸的证据。
DOI：
10.1021/bi00281a016
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发表时间：
1983
期刊：
影响因子：
2.9
通讯作者：
Weinstein,B
中科院分区：
文献类型：
--
作者：
McMullen,BA;Fujikawa,K;Kisiel,W;Sasagawa,T;Howald,WN;Kwa,EY;Weinstein,B
材料和方法羧肽酶A和B、TPCK-胰蛋白酶和α-胰凝乳蛋白酶从Worthington获得。在使用前，在苯甲脒-琼脂糖上进一步纯化TPCK-胰蛋白酶和α-胰凝乳蛋白酶（Fujikawa& McMullen，1983）。碘乙酸购自Sigma，氨肽酶购自Boehringer曼海姆。L-[2，3- 3 H2]天冬氨酸得自新英格兰核公司。L-苏型-β-羟基-天冬氨酸和L-苏型-β-羟基-天冬氨酸由N. Izumiya和T. Kato，Faculty of Biochemistry，Kyushu University，Fukuoka，Japan（Okai等人，
Materials and MethodsCarboxypeptidases A and B, TPCK-trypsin, and a-chymotrypsin were obtained from Worthington. Before use, the TPCK-trypsin and a-chymotrypsin were purified further on benzamidine-agarose (Fujikawa& McMullen, 1983). Io-doacetic acid was purchased from Sigmaand aminopeptidase from Boehringer Mannheim. L-[2, 3-3H2] Aspartic acid was obtained from New England Nuclear. L-threo-ft-Hydroxy-aspartic acid and Le/^ r/iro-/3-hydroxyaspartic acid were kindly provided by Drs. N. Izumiya and T. Kato, Faculty of Biochemistry, Kyushu University, Fukuoka, Japan (Okai et al.,