Construction of pH-sensitive lysozyme/pectin nanogel for tumor methotrexate delivery
Construction of pH-sensitive lysozyme/pectin nanogel for tumor methotrexate delivery
复制标题
用于肿瘤甲氨蝶呤递送的 pH 敏感溶菌酶/果胶纳米凝胶的构建
DOI：
10.1016/j.colsurfb.2014.12.051
复制
发表时间：
2015-02-01
影响因子：
5.8
通讯作者：
Chen, Yijie
中科院分区：
文献类型：
--
作者：
Lin, Liufeng;Xu, Wei;Chen, Yijie
以溶菌酶-果胶为原料，通过自组装制备了新型纳米粒子，并将其作为抗肿瘤药物甲氨蝶呤（MTX）的载体。纳米凝胶呈现球形，直径约为109 +/- 2 nm，粒径分布窄，表面带负电荷。此外，MTX的掺入几乎没有改变纳米凝胶的粒径和形貌。MTX在纳米凝胶中的载药量可达17.58 ± 0.85%。MTX负载的纳米凝胶是pH依赖性的，在pH从7.4降低到5.3时加速释放MTX。MU测定表明包封MTX比游离MTX表现出更高的抗癌活性。同时，载MTX的纳米凝胶可以被HepG 2细胞有效地内吞，导致与游离MTX相比增强的癌细胞凋亡。结果表明，该纳米凝胶具有良好的生物相容性和低毒性。所得纳米凝胶在开发新型抗癌药物纳米载体方面具有很大的潜力。(C)2015爱思唯尔B. V.保留所有权利。
Novel nano-particles were developed from lysozyme-pectin through self-assembly, and the nanogels could be used as a carrier for the antitumor agent, methotrexate (MTX). The nanogels exhibited spherical with diameters about 109 +/- 2 nm and narrow particle size distribution, as well as negative surface charge. Furthermore, the particle size and morphology of the nanogels hardly changed with the incorporation of MTX. The loading capacity of MTX in nanogels could reach 17.58 +/- 0.85%. MTX-loaded nanogels were pH-dependent, accelerated release of MTX at a decreasing pH from 7.4 to 5.3. The MU assay indicated that encapsulated MTX exhibited higher anticancer activity than free MTX. Meanwhile, MTX-loaded nanogels could be effectively endocytosed by HepG2 cells, resulting in enhanced cancer-cell apoptosis comparing to free MTX. It indicated that the nanogels had good biocompatibility and low toxicity. The obtained nanogels had great potential in the development of a new nanocarrier for anti-cancer drug delivery. (C) 2015 Elsevier B.V. All rights reserved.