Establishment of Reference Ranges for Cortisol in Neonates, Infants, Children and Adolescents
Establishment of Reference Ranges for Cortisol in Neonates, Infants, Children and Adolescents
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新生儿、婴儿、儿童和青少年皮质醇参考范围的建立
DOI：
10.1515/cclm.1993.31.8.525
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
G. Wiedemann
中科院分区：
文献类型：
--
作者：
L. Jonetz;G. Wiedemann
使用Abbott Laboratories的TDx荧光偏振免疫测定法（FPIA）测定了687名年龄在5天至18岁之间（新生儿、婴儿、儿童和青少年）的正常健康先证者血清中的皮质醇。先证者群体分为9个年龄组，每组分为男性和女性。根据国际临床化学联合会的建议，将95%散射范围作为参考范围。只有少数参考组显示正态高斯分布。除第50百分位数外，还确定了所有参考组的第2.5和第97.5百分位数。还测定了最小值和最大值。使用Mann & Whitney的U检验来检验各个参考组之间的显著差异。将显示无显著差异的组合并，并最终计算正常、健康新生儿、婴儿、儿童和青少年血清中皮质醇的参考范围。血清皮质醇浓度随年龄增长而显著升高。在14-15岁年龄组中，男女之间也存在显著差异。
Cortisol was determined in the sera of 687 normal, healthy probands between the ages of 5 days and 18 years (neonates, infants, children and adolescents), using a fluorescence polarization immunoassay (FPIA) on the TDx from Abbott Laboratories. The proband collective was divided into 9 age groups and each group into males and females. In accordance with the recommendations of the International Federation of Clinical Chemistry, the 95% scatter range was taken as the reference range. Only a few reference groups showed a normal Gaussian distribution. In addition to the 50th percentile, the 2.5th and 97.5th percentiles were determined for all reference groups. Minimal and maximal values were also determined. The U-test of Mann & Whitney was used to test for significant differences between the individual reference groups. Groups showing no significant differences were combined, and reference ranges were finally calculated for cortisol in the serum of normal, healthy neonates, infants, children and adolescents. The serum cortisol concentration showed a significant increase with age. In the 14-15 years age group, there was also a significant difference between males and females.