Renal ischemia-induced increase in vascular permeability is limited by hypothermia
Renal ischemia-induced increase in vascular permeability is limited by hypothermia
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DOI：
10.1016/s0162-3109(99)00095-8
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发表时间：
1999-09-01
期刊：
影响因子：
--
通讯作者：
Catanzaro, OL
中科院分区：
文献类型：
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作者：
Carattino, MD;Cueva, F;Catanzaro, OL
本工作的目的是评价激肽释放酶-激动素系统和低温在肾缺血再灌注过程中的作用。雄性C57BL/KSJmdb小鼠分别于缺血20分钟和60分钟后进行不同时间的再灌流。我们的结果表明，短时间的缺血再灌注没有引起激肽释放酶活性、伊文思蓝(EB)渗出、激肽释放酶原、髓过氧化物酶活性或血浆肌酐浓度的显著变化。治疗组小鼠在再灌流1h时出现明显的水肿，但在再灌流24 h后恢复到基础值。缺血60min小鼠的激肽释放酶活性和EB渗出明显增加。缺血60min再灌注24 h组小鼠肾脏髓过氧化物酶活性呈网状分布。从肾脏和浸润性白细胞中释放出来的组织激肽释放酶产生激动素和激活基质降解酶，可能至少是造成小鼠肾脏缺血再灌注60min损伤的部分原因。低温显著减轻了60min缺血小鼠的炎症过程，并确实阻止了血管通透性的增加。然而，在常温和低温缺血小鼠之间，组织水肿并没有显示出改变。(C)1999 Elsevier Science B.V.保留所有权利。
The purpose of the present work was to evaluate the kallikrein-kinin system and effects of hypothermia during renal ischemia and reperfusion. Male C57BL/KSJmdb mice were subjected to 20 or 60 min ischemia for different periods of reperfusion. Our results demonstrate that short periods of ischemia followed by reperfusion did nor cause significant alterations in kallikrein activity, Evans Blue (EB) extravasation, prokallikreins, myeloperoxidase activity or plasma creatinine concentration. Edema was evident at 1 h reperfusion in the treated mice, but returned to basal values after 24 h reperfusion. Kallikrein activities and EB extravasation showed a significant increase in 60 min ischemic mice. Myeloperoxidase activity in the kidney of the mice confirmed net infiltration in the group with 60 min ischemia and 24 h reperfusion. The generation of kinins and activation of matrix degrading enzymes by tissue kallikrein, liberated from both renal and infiltrated leukocytes, could be responsible at least in part for the damage observed in the kidney of mice subject to 60 min ischemia and reperfusion. The hypothermia significantly reduced the inflammatory process in the 60 min ischemic mice, and did prevent an increase in vascular permeability. Nevertheless, the tissue edema was not shown to change between normothermic and hypothermic ischemic mice. (C) 1999 Elsevier Science B.V. All rights reserved.