RNA polymerase II targets pre-mRNA splicing factors to transcription sites in vivo
RNA polymerase II targets pre-mRNA splicing factors to transcription sites in vivo
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DOI：
10.1016/s1097-2765(01)80002-2
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发表时间：
1999-06-01
期刊：
影响因子：
16
通讯作者：
Spector, DL
中科院分区：
文献类型：
--
作者：
Misteli, T;Spector, DL
生物化学证据表明，前mRNA剪接因子与RNA聚合酶II最大亚基的C-末端结构域物理相互作用。我们已经研究了这种相互作用的体内功能。在哺乳动物细胞中，RNA pol II LS的CTD的截短防止剪接机器靶向转录位点。在不存在CID的情况下，前体mRNA剪接严重减少。单独存在未剪接的RNA不足以使剪接因子在转录位点积累，也不足以使其有效剪接。我们的研究结果表明，CTD的RNA聚合酶II LS在核内靶向剪接因子的转录位点在体内的关键作用。
Biochemical evidence indicates that pre-mRNA splicing factors physically interact with the C-terminal domain of tt le largest subunit of RNA polymerase II. We have investigated the in vivo function of this interaction. In mammalian cells, truncation of the CTD of RNA pol II LS prevents the targeting of the splicing machinery to a transcription site. In the absence of the CID, pre-mRNA splicing is severely reduced. The presence of unspliced RNA alone is not sufficient for the accumulation of splicing factors at the transcription site, nor for its efficient splicing. Our results demonstrate a critical role for the CTD of RNA pol II LS in the intranuclear targeting of splicing factors to transcription sites in vivo.