Kaposi's sarcoma patients in the Swiss HIV Cohort Study with and without detectable human herpesvirus 8 in peripheral blood mononuclear cells.
Kaposi's sarcoma patients in the Swiss HIV Cohort Study with and without detectable human herpesvirus 8 in peripheral blood mononuclear cells.
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瑞士 HIV 队列研究中的卡波西肉瘤患者外周血单核细胞中检测到或未检测到人类疱疹病毒 8。
DOI：
10.1097/00002030-199715000-00021
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
Vernazza,PL
中科院分区：
文献类型：
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作者：
Quinlivan,EB;Ye,D;Wehbie,RS;Stewart,PW;Flepp,M;Vernazza,PL
人疱疹病毒（HHV）-8 DNA可在部分但并非所有卡波西肉瘤（KS）患者的外周血样本中检出[1，2]。由于HHV-8存在于几乎所有KS病变中[3-7]，因此尚不清楚为什么只有50%的患者外周血单核细胞（PBMC）中存在可检测量的HHV-8 DNA。我们使用聚合酶链反应（PCR）检测瑞士HIV队列中KS患者PBMC中的HHV-8 DNA，以解决该问题[8]。使用巢式次要衣壳和蛋白酶PCR测定法对储存的系列PBMC进行PCR（图1a）[9]。用6× 10 4 PBMC的DNA检测11份标本可检出HHV 8 DNA。在将输入DNA增加三倍后，4份样品具有可检测的HHV-8 DNA。一份样本（患者4）通过次要衣壳PCR检测重复呈阳性，但蛋白酶PCR检测无法确认结果。因此，20例KS患者中有9例（45%）在外周血中可检测到HHV-8 DNA。在这9例患者中，8例在初始样本访视时呈阳性（中位数，KS诊断前15个月）。唯一一名有记录的HHV-8 DNA从不可检测到可检测的转换的患者在KS诊断前11-18个月这样做。
Human herpesvirus (HHV)-8 DNA is detectable in peripheral blood samples of some but not all patients with Kaposi's sarcoma (KS)[1, 2]. Because HHV-8 is present in virtually all KS lesions [3–7], it is not clear why only 50% of the patients have detectable amounts of HHV-8 DNA in peripheral blood mononuclear cells (PBMC). We used polymerase chain reaction (PCR) to detect HHV-8 DNA in PBMC from KS patients in the Swiss HIV Cohort to address this question [8].PCR was performed on stored serial PBMC (Fig. 1a) using nested minor capsid and protease PCR assays [9]. Eleven samples had detectable HHV-8 DNA using DNA from 6× 10 4 PBMC. Four samples had detectable HHV-8 DNA after increasing the input DNA threefold. One sample (patient 4) was repeatedly positive by minor capsid PCR assay, although the protease PCR assay could not confirm the results. Therefore, nine (45%) of the 20 patients with KS had HHV-8 DNA that was detectable in their peripheral blood. Of these nine patients, eight were positive at the initial sample visit (median, 15 months prior to KS diagnosis). The only patient with a documented conversion from undetectable to detectable HHV-8 DNA did so 11–18 months before the diagnosis of KS.