Overexpression of receptor-like kinase ERECTA improves thermotolerance in rice and tomato
Overexpression of receptor-like kinase ERECTA improves thermotolerance in rice and tomato
复制标题
受体样激酶 ERECTA 的过度表达可提高水稻和番茄的耐热性
DOI：
10.1038/nbt.3321
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发表时间：
2015-09-01
影响因子：
46.9
通讯作者：
He, Zuhua
中科院分区：
文献类型：
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作者：
Shen, Hui;Zhong, Xiangbin;He, Zuhua
全球变暖对农作物生产力的不利影响可能会减少世界粮食供应。虽然已经研究了植物对温度变化的反应，但迄今为止，对作物进行基因改造以提高耐热性几乎没有成功。在这里，我们证明了拟南芥受体样激酶ERECTA(ER)在拟南芥、水稻和番茄中的过表达赋予了不依赖于水分损失的耐热性，并且拟南芥ER突变体对热高度敏感。水稻ER同源物的功能丧失突变和番茄ER等位基因的表达减少降低了两个物种的耐热性。在中国的温室和田间多个季节的试验中，高表达拟南芥ER的转基因番茄和水稻品系表现出较好的耐热性。此外，高表达ER的拟南芥、番茄和水稻植株的生物量也有所增加。我们的发现可能有助于在不损害生长的情况下设计或培育耐热作物。
The detrimental effects of global warming on crop productivity threaten to reduce the world's food supply. Although plant responses to changes in temperature have been studied, genetic modification of crops to improve thermotolerance has had little success to date. Here we demonstrate that overexpression of the Arabidopsis thaliana receptor-like kinase ERECTA (ER) in Arabidopsis, rice and tomato confers thermotolerance independent of water loss and that Arabidopsis er mutants are hypersensitive to heat. A loss-of-function mutation of a rice ER homolog and reduced expression of a tomato ER allele decreased thermotolerance of both species. Transgenic tomato and rice lines overexpressing Arabidopsis ER showed improved heat tolerance in the greenhouse and in field tests at multiple locations in China during several seasons. Moreover, ER-overexpressing transgenic Arabidopsis, tomato and rice plants had increased biomass. Our findings could contribute to engineering or breeding thermotolerant crops with no growth penalty.