Expression of CD133+ cancer stem cells in childhood malignant melanoma and its correlation with metastasis
Expression of CD133+ cancer stem cells in childhood malignant melanoma and its correlation with metastasis
复制标题
DOI：
10.1038/modpathol.2009.163
复制
发表时间：
2010-03-01
期刊：
影响因子：
7.5
通讯作者：
Kokta, Victor
中科院分区：
文献类型：
--
作者：
Al Dhaybi, Rola;Sartelet, Herve;Kokta, Victor
表达CD133的肿瘤干细胞广泛存在于多种肿瘤中，在恶性黑色素瘤中发现它们可能有助于改进分类、诊断和治疗。为了研究CD133在儿童黑色素瘤中的表达与淋巴结和/或内脏转移的关系，我们评估了12例恶性黑色素瘤和12例儿童Spitz痣的对照。CD133阳性病例行CD133和Ki-67双免疫染色。3例黑色素瘤患者有淋巴结转移，1例有多脏器转移；CD133仅在这4例患者中呈阳性。在三例病例中，CD133（+）细胞的Ki-67指数低于CD133（-）黑色素瘤细胞。我们在所有的斯皮茨痣中未发现CD133阳性。CD133（+）癌干细胞在儿童恶性黑色素瘤中的表达可能与淋巴结和/或内脏转移有关，并且可能具有低增殖Ki-67指数，这可能解释了它们的化疗耐药。现代病理学（2010）23,376 -380；doi: 10.1038 / modpathol.2009.163;2010年1月8日在线发布
Cancer stem cells expressing CD133 exist in a wide array of tumors and their identification in malignant melanoma may help refine classification, diagnosis and treatment. To study the correlation between CD133 expression in childhood melanoma and lymph node and/or visceral metastasis, we evaluated 12 cases of malignant melanoma and 12 control cases of Spitz nevus occurring in children. Double immunostaining with CD133 and Ki-67 was performed in the cases showing CD133 positivity. Three melanoma patients had lymph node metastasis and only one had multivisceral metastases; CD133 was positive only in these four patients. The Ki-67 index was lower in the CD133(+) cells in comparison with the CD133(-) melanoma cells in three cases. We found no positivity for CD133 in all the Spitz nevi. CD133(+) cancer stem cell expression in childhood malignant melanoma might correlate with lymph node and/or visceral metastasis and may have a low proliferative Ki-67 index that might explain their chemoresistance. Modern Pathology (2010) 23, 376-380; doi: 10.1038/modpathol.2009.163; published online 8 January 2010