Glycan microarrays: Powerful tools for biomarker discovery
Glycan microarrays: Powerful tools for biomarker discovery
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DOI：
10.3233/cbm-130383
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发表时间：
2014-01-01
影响因子：
3.1
通讯作者：
Gildersleeve, Jeffrey C.
中科院分区：
文献类型：
--
作者：
Muthana, Saddam M.;Gildersleeve, Jeffrey C.
在过去的10年里，聚糖微阵列技术已经成为一种强大的高通量工具，用于研究碳水化合物与各种生物分子的相互作用。阵列格式允许一个屏幕上千的结合相互作用，在一个单一的实验中使用最少量的稀缺材料。最近，这项技术已被应用于发现诊断，预后，风险预测和监测免疫反应的生物标志物。使用聚糖阵列的生物标志物发现主要集中在监测血清中抗聚糖抗体库的变化，因为抗体群体可随着疾病发作、暴露于病原体或接种疫苗而显著变化。在此，我们回顾了使用聚糖阵列来识别癌症、感染、自身免疫性疾病和免疫反应的新生物标志物的努力。
Over the last 10 years, glycan microarray technology has emerged as a powerful high-throughput tool for studying the interactions of carbohydrates with a variety of biomolecules. The array format allows one to screen thousands of binding interactions in a single experiment using minimal amounts of scarce materials. More recently, this technology has been applied to the discovery of biomarkers for diagnosis, prognosis, risk prediction, and monitoring immune responses. Biomarker discovery using glycan arrays has primarily focused on monitoring changes to the anti-glycan antibody repertoires in serum, since the populations of antibodies can change significantly with the onset of disease, exposure to pathogens, or vaccination. Herein, we review efforts to use glycan arrays to identify new biomarkers for cancer, infections, autoimmune diseases, and immune responses.